BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18819

Title: 1H, 13C and 15N assignments of the Apo-acyl carrier protein-3 of Pseudomonas aeruginosa.   PubMed: 21152998

Deposition date: 2012-11-05 Original release date: 2012-12-04

Authors: Parnham, Stuart; Duggan, Brendan; Roca, Amancio; Zhang, Yong-Mei

Citation: Parnham, Stuart; Gaines, William; Duggan, Brendan; Marcotte, William; Hennig, Mirko. "NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1."  Biomol. NMR Assignments 5, 131-133 (2011).

Assembly members:
ApoAcpP-3, polymer, 82 residues, Formula weight is not available

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ApoAcpP-3: GSHMPNDMEDHLLTVLSVAS GVPKEEISRDSRMEDLAFDS LVVSELSLKLRKEFGVTGVD DELDLLETVDELFQLVEKHR AA

Data sets:
Data typeCount
13C chemical shifts349
15N chemical shifts81
1H chemical shifts555

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ApoAcpP-31

Entities:

Entity 1, ApoAcpP-3 82 residues - Formula weight is not available

1   GLYSERHISMETPROASNASPMETGLUASP
2   HISLEULEUTHRVALLEUSERVALALASER
3   GLYVALPROLYSGLUGLUILESERARGASP
4   SERARGMETGLUASPLEUALAPHEASPSER
5   LEUVALVALSERGLULEUSERLEULYSLEU
6   ARGLYSGLUPHEGLYVALTHRGLYVALASP
7   ASPGLULEUASPLEULEUGLUTHRVALASP
8   GLULEUPHEGLNLEUVALGLULYSHISARG
9   ALAALA

Samples:

15N-ApoAcpP-3: ApoAcpP-3, [U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

13C15N-ApoAcpP-3: ApoAcpP-3, [U-99% 13C; U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N-ApoAcpP-3isotropicsample_conditions_1
3D CBCA(CO)NH13C15N-ApoAcpP-3isotropicsample_conditions_1
3D HNCACB13C15N-ApoAcpP-3isotropicsample_conditions_1
3D HNCO13C15N-ApoAcpP-3isotropicsample_conditions_1
3D HBHA(CO)NH13C15N-ApoAcpP-3isotropicsample_conditions_1
3D H(CCO)NH13C15N-ApoAcpP-3isotropicsample_conditions_1
3D HCCH-TOCSY13C15N-ApoAcpP-3isotropicsample_conditions_1
2D 1H-13C HSQC aliphatic13C15N-ApoAcpP-3isotropicsample_conditions_1
2D 1H-13C HSQC aromatic13C15N-ApoAcpP-3isotropicsample_conditions_1
3D 1H-15N NOESY15N-ApoAcpP-3isotropicsample_conditions_1
3D 1H-13C NOESY aliphatic13C15N-ApoAcpP-3isotropicsample_conditions_1
3D 1H-13C NOESY aromatic13C15N-ApoAcpP-3isotropicsample_conditions_1

Software:

CcpNMR v2.2.2, CCPN - chemical shift assignment

TOPSPIN v3.0.4, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 850 MHz

Related Database Links:

BMRB 18468
PDB
DBJ BAK91288 BAP22043 BAP49673 BAQ38558 BAR66624
EMBL CAW26458 CCQ85541 CDH69992 CDH76078 CDI91738
GB AAG06722 ABJ12585 AEO74118 AFM63813 AGI80541
REF NP_252024 WP_003091713 WP_003106204 WP_034068812 WP_039700511

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts