BMRB Entry 26640
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26640
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H, 15N and 13C chemical shift assignment of the alpha-crystallin domain and the C-terminal domain in human alpha-B crystallin oligomers. PubMed: 26458046
Deposition date: 2015-08-26 Original release date: 2015-09-28
Authors: Mainz, Andi; Reif, Bernd
Citation: Mainz, Andi; Peschek, Jirka; Stavropoulou, Maria; Back, Katrin; Asami, Sam; Bardiaux, Benjamin; Prade, Elke; Peters, Carsten; Weinkauf, Sevil; Buchner, Johannes; Reif, Bernd. "The chaperone AlphaB-crystallin uses different interfaces to capture an amorphous and an amyloid client" Nat. Struct. Mol. Biol. 22, 898-905 (2015).
Assembly members:
aB, polymer, 175 residues, 20159 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
aB: MDIAIHHPWIRRPFFPFHSP
SRLFDQFFGEHLLESDLFPT
STSLSPFYLRPPSFLRAPSW
FDTGLSEMRLEKDRFSVNLD
VKHFSPEELKVKVLGDVIEV
HGKHEERQDEHGFISREFHR
KYRIPADVDPLTITSSLSSD
GVLTVNGPRKQVSGPERTIP
ITREEKPAVTAAPKK
Data type | Count |
13C chemical shifts | 240 |
15N chemical shifts | 100 |
1H chemical shifts | 101 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | alpha B crystallin, 1 | 1 |
2 | alpha B crystallin, 2 | 1 |
3 | alpha B crystallin, 3 | 1 |
4 | alpha B crystallin, 4 | 1 |
5 | alpha B crystallin, 5 | 1 |
6 | alpha B crystallin, 6 | 1 |
7 | alpha B crystallin, 7 | 1 |
8 | alpha B crystallin, 8 | 1 |
9 | alpha B crystallin, 9 | 1 |
10 | alpha B crystallin, 10 | 1 |
11 | alpha B crystallin, 11 | 1 |
12 | alpha B crystallin, 12 | 1 |
13 | alpha B crystallin, 13 | 1 |
14 | alpha B crystallin, 14 | 1 |
15 | alpha B crystallin, 15 | 1 |
16 | alpha B crystallin, 16 | 1 |
17 | alpha B crystallin, 17 | 1 |
18 | alpha B crystallin, 18 | 1 |
19 | alpha B crystallin, 19 | 1 |
20 | alpha B crystallin, 20 | 1 |
21 | alpha B crystallin, 21 | 1 |
22 | alpha B crystallin, 22 | 1 |
23 | alpha B crystallin, 23 | 1 |
24 | alpha B crystallin, 24 | 1 |
25 | alpha B crystallin, 25 | 1 |
26 | alpha B crystallin, 26 | 1 |
27 | alpha B crystallin, 27 | 1 |
28 | alpha B crystallin, 28 | 1 |
Entities:
Entity 1, alpha B crystallin, 1 175 residues - 20159 Da.
1 | MET | ASP | ILE | ALA | ILE | HIS | HIS | PRO | TRP | ILE | ||||
2 | ARG | ARG | PRO | PHE | PHE | PRO | PHE | HIS | SER | PRO | ||||
3 | SER | ARG | LEU | PHE | ASP | GLN | PHE | PHE | GLY | GLU | ||||
4 | HIS | LEU | LEU | GLU | SER | ASP | LEU | PHE | PRO | THR | ||||
5 | SER | THR | SER | LEU | SER | PRO | PHE | TYR | LEU | ARG | ||||
6 | PRO | PRO | SER | PHE | LEU | ARG | ALA | PRO | SER | TRP | ||||
7 | PHE | ASP | THR | GLY | LEU | SER | GLU | MET | ARG | LEU | ||||
8 | GLU | LYS | ASP | ARG | PHE | SER | VAL | ASN | LEU | ASP | ||||
9 | VAL | LYS | HIS | PHE | SER | PRO | GLU | GLU | LEU | LYS | ||||
10 | VAL | LYS | VAL | LEU | GLY | ASP | VAL | ILE | GLU | VAL | ||||
11 | HIS | GLY | LYS | HIS | GLU | GLU | ARG | GLN | ASP | GLU | ||||
12 | HIS | GLY | PHE | ILE | SER | ARG | GLU | PHE | HIS | ARG | ||||
13 | LYS | TYR | ARG | ILE | PRO | ALA | ASP | VAL | ASP | PRO | ||||
14 | LEU | THR | ILE | THR | SER | SER | LEU | SER | SER | ASP | ||||
15 | GLY | VAL | LEU | THR | VAL | ASN | GLY | PRO | ARG | LYS | ||||
16 | GLN | VAL | SER | GLY | PRO | GLU | ARG | THR | ILE | PRO | ||||
17 | ILE | THR | ARG | GLU | GLU | LYS | PRO | ALA | VAL | THR | ||||
18 | ALA | ALA | PRO | LYS | LYS |
Samples:
sample_1: aB, [U-13C; U-15N; U-2H], 2 mM; sodium phosphate 50 mM; sodium chloride 100 mM
sample_2: aB, [U-13C; U-15N; U-2H], 25 mM; sodium phosphate 50 mM; sodium chloride 100 mM; Cu(II)-EDTA 60 mM
sample_3: aB, [U-13C; U-15N; U-2H], 25 mM; sodium phosphate 50 mM; sodium chloride 100 mM; Cu(II)-EDTA 60 mM
sample_conditions_1: pH: 7.5; temperature: 295 K
sample_conditions_2: pH: 7.5; temperature: 277 K
sample_conditions_3: pH: 7.5; temperature: 289 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N CP | sample_2 | anisotropic | sample_conditions_2 |
3D hCAhNH | sample_2 | anisotropic | sample_conditions_2 |
3D hCOhNH | sample_2 | anisotropic | sample_conditions_2 |
2D 1H-15N CP | sample_3 | anisotropic | sample_conditions_3 |
3D RFDR-HSQC HHN | sample_3 | anisotropic | sample_conditions_3 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 750 MHz
- Bruker Avance 600 MHz
- Bruker Avance 900 MHz
Related Database Links:
PDB | |
BMRB | 16391 25527 |
DBJ | BAD51947 BAE27257 BAE40798 BAE87237 BAG36739 |
EMBL | CAA42910 CAA42911 CAA64669 CAC33095 CAF02108 |
GB | AAA03655 AAA37472 AAA40977 AAA52104 AAA67045 |
PIR | I53319 |
PRF | 2015215A |
REF | NP_001012475 NP_001075876 NP_001125917 NP_001247830 NP_001271991 |
SP | P02510 P02511 P23927 P23928 P41316 |
TPG | DAA22360 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts