BMRB Entry 4050
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4050
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Title: 1H, 13C, 15N Chemical Shift Assignments for Reduced Clostridium Pasteurianum Rubredoxin
Deposition date: 1997-08-01 Original release date: 1999-08-15
Authors: Prantner, Andrew; Volkman, Brian; Wilkens, Steven; Xia, Bin; Markley, John
Citation: Prantner, Andrew; Volkman, Brian; Wilkens, Steven; Xia, Bin; Markley, John. "Assignment of 1H, 13C, 15N Signals of Reduced Clostridium Pasteurianum Rubredoxin: Oxidation State-Dependent Changes in Chemical Shifts and Relaxation Rates" J. Biomol. NMR 10, 411-412 (1997).
Assembly members:
rubredoxin peptide, polymer, 54 residues, 6040 Da.
FE2, non-polymer, 55.845 Da.
Natural source: Common Name: C. pasteurianum Taxonomy ID: 1501 Superkingdom: Eubacteria Kingdom: Monera Genus/species: Clostridium pasteurianum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
rubredoxin peptide: MKKYTCTVCGYIYNPEDGDP
DNGVNPGTDFKDIPDDWVCP
LCGVGKDQFEEVEE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 150 |
15N chemical shifts | 41 |
1H chemical shifts | 235 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | rubredoxin | 1 |
2 | Fe(II) | 2 |
Entities:
Entity 1, rubredoxin 54 residues - 6040 Da.
1 | MET | LYS | LYS | TYR | THR | CYS | THR | VAL | CYS | GLY | ||||
2 | TYR | ILE | TYR | ASN | PRO | GLU | ASP | GLY | ASP | PRO | ||||
3 | ASP | ASN | GLY | VAL | ASN | PRO | GLY | THR | ASP | PHE | ||||
4 | LYS | ASP | ILE | PRO | ASP | ASP | TRP | VAL | CYS | PRO | ||||
5 | LEU | CYS | GLY | VAL | GLY | LYS | ASP | GLN | PHE | GLU | ||||
6 | GLU | VAL | GLU | GLU |
Entity 2, Fe(II) - Fe - 55.845 Da.
1 | FE2 |
Samples:
sample_one: Rubredoxin4 6 mM; Phosphate buffer mM
sample_conditions_one: pH: 6.0 na; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
not available | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
BMRB | 4051 4066 4137 4182 4319 4320 |
PDB | |
GB | AAA23279 AJA49845 AJA53833 ELP57804 KER11884 |
REF | WP_003447684 |
SP | P00268 |
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