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BMRB Entry 4368

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4368

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Title: Chemical Shift Assignments for A35T vnd/NK2 Mutant Homeodomain

Deposition date: 1999-07-14 Original release date: 2000-06-29

Authors: Xiang, Bosong; Weiler, S.; Nirenberg, M; Ferretti, J.

Citation: Xiang, Bosong; Weiler, S.; Nirenberg, M; Ferretti, J.. "Structural Basis of an Embryonically Lethal Single Ala --> Thr Mutation in the vnd/NK-2 Homeodomain"  Proc. Natl. Acad. Sci. U. S. A. 95, 7412-7416 (1998).

Assembly members:
A35T vnd/NK2 homeodomain, polymer, 80 residues, 9723.7 Da.
strand 1, polymer, 16 residues, Formula weight is not available
strand 2, polymer, 16 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7215   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: drosophila not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia Coli

Entity Sequences (FASTA):
A35T vnd/NK2 homeodomain: GSHMSDGLPNKKRKRRVLFT KAQTYELERRFRQQRYLSAP EREHLTSLIRLTPTQVKIWF QNHRYKTKRAQNEKGYEGHP
strand 1: ACAGCCACTTGACACA
strand 2: TGTGTCAAGTGGCTGT

Data sets:
Data typeCount
13C chemical shifts265
15N chemical shifts76
1H chemical shifts549

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A35T vnd/NK21
2strand 12
3strand 23

Entities:

Entity 1, A35T vnd/NK2 80 residues - 9723.7 Da.

1   GLYSERHISMETSERASPGLYLEUPROASN
2   LYSLYSARGLYSARGARGVALLEUPHETHR
3   LYSALAGLNTHRTYRGLULEUGLUARGARG
4   PHEARGGLNGLNARGTYRLEUSERALAPRO
5   GLUARGGLUHISLEUTHRSERLEUILEARG
6   LEUTHRPROTHRGLNVALLYSILETRPPHE
7   GLNASNHISARGTYRLYSTHRLYSARGALA
8   GLNASNGLULYSGLYTYRGLUGLYHISPRO

Entity 2, strand 1 16 residues - Formula weight is not available

1   DADCDADGDCDCDADCDTDT
2   DGDADCDADCDA

Entity 3, strand 2 16 residues - Formula weight is not available

1   DTDGDTDGDTDCDADADGDT
2   DGDGDCDTDGDT

Samples:

15N-A35T: A35T vnd/NK2 homeodomain, [U-15N], 0.7 mM; H2O 90%; D2O 10%

15N_13C-A35T_H2O: A35T vnd/NK2 homeodomain, [U-15N; U-13C], 0.7 mM; strand 1 0.7 mM; strand 2 0.7 mM

15N_13C-A35T_D2O: A35T vnd/NK2 homeodomain, [U-15N; U-13C], 0.7 mM; strand 1 0.7 mM; strand 2 0.7 mM

A35T_DNA_conditions: pH: 6.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-15N HMQCnot availablenot availablenot available
3D CBCANHnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
3D 1H-15N-1H NOESY-HSQCnot availablenot availablenot available
4D 1H-13C-15N-1H HMQC-NOESY-HSQCnot availablenot availablenot available
4D 1H-13C-13C-1H HMQC-NOESY-HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 4141
PDB
EMBL CAA21410 CAA60619
GB AAA28617 AAB34960 AAF45521 ABI30961 ADJ13174
REF NP_001036253 NP_476786 XP_001354499 XP_001966310 XP_001982405
SP P22808

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