BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5287

Title: Backbone 1H and 15N Chemical shift assigments for [2H,15N]CaM:CaMKI(320) complex   PubMed: 11904288

Deposition date: 2002-02-12 Original release date: 2002-06-13

Authors: Kranz, James; Lee, Eun; Nairn, Angus; Wand, A.

Citation: Kranz, James; Lee, Eun; Nairn, Angus; Wand, A.. "A Direct Test of the Reductionist Approach to Structural Studies of Calmodulin Activity: Relevance of Peptide Models of Target Proteins"  J. Biol. Chem. 277, 16351-16354 (2002).

Assembly members:
calmodulin, polymer, 148 residues, 16706 Da.
Ca2+/CaM-dependent (rat) protein kinase I, polymer, 340 residues, 36000 Da.

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
Ca2+/CaM-dependent (rat) protein kinase I: MGSSHHHHHHSSGLVPRGSH MPGAVEGPRWKQAEDIRDIY DFRDVLGTGAFSEVILAEDK RTQKLVAIKCIAKKALEGKE GSMENEIAVLHKIKHPNIVA LDDIYESGGHLYLIMQLVSG GELFDRIVEKGFYTERDASR LIFQVLDAVKYLHDLGIVHR DLKPENLLYYSLDEDSKIMI SDFGLSKMEDPGSVLSTACG TPGYVAPEVLAQKPYSKAVD CWSIGVIAYILLCGYPPFYD ENDAKLFEQILKAEYEFDSP YWDDISDSAKDFIRHLMEKD PEKRFTCEQALQHPWIAGDT ALDKNIHQSVSEQIKKNFAK SKWKQAFNATAVVRHMRKLQ

Data sets:
Data typeCount
1H chemical shifts144
15N chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2CaM-dependent kinase I (1-320)2

Entities:

Entity 1, calmodulin 148 residues - 16706 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, CaM-dependent kinase I (1-320) 340 residues - 36000 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METPROGLYALAVALGLUGLYPROARGTRP
4   LYSGLNALAGLUASPILEARGASPILETYR
5   ASPPHEARGASPVALLEUGLYTHRGLYALA
6   PHESERGLUVALILELEUALAGLUASPLYS
7   ARGTHRGLNLYSLEUVALALAILELYSCYS
8   ILEALALYSLYSALALEUGLUGLYLYSGLU
9   GLYSERMETGLUASNGLUILEALAVALLEU
10   HISLYSILELYSHISPROASNILEVALALA
11   LEUASPASPILETYRGLUSERGLYGLYHIS
12   LEUTYRLEUILEMETGLNLEUVALSERGLY
13   GLYGLULEUPHEASPARGILEVALGLULYS
14   GLYPHETYRTHRGLUARGASPALASERARG
15   LEUILEPHEGLNVALLEUASPALAVALLYS
16   TYRLEUHISASPLEUGLYILEVALHISARG
17   ASPLEULYSPROGLUASNLEULEUTYRTYR
18   SERLEUASPGLUASPSERLYSILEMETILE
19   SERASPPHEGLYLEUSERLYSMETGLUASP
20   PROGLYSERVALLEUSERTHRALACYSGLY
21   THRPROGLYTYRVALALAPROGLUVALLEU
22   ALAGLNLYSPROTYRSERLYSALAVALASP
23   CYSTRPSERILEGLYVALILEALATYRILE
24   LEULEUCYSGLYTYRPROPROPHETYRASP
25   GLUASNASPALALYSLEUPHEGLUGLNILE
26   LEULYSALAGLUTYRGLUPHEASPSERPRO
27   TYRTRPASPASPILESERASPSERALALYS
28   ASPPHEILEARGHISLEUMETGLULYSASP
29   PROGLULYSARGPHETHRCYSGLUGLNALA
30   LEUGLNHISPROTRPILEALAGLYASPTHR
31   ALALEUASPLYSASNILEHISGLNSERVAL
32   SERGLUGLNILELYSLYSASNPHEALALYS
33   SERLYSTRPLYSGLNALAPHEASNALATHR
34   ALAVALVALARGHISMETARGLYSLEUGLN

Samples:

sample_1: calmodulin, [U-99% 2H; U-99% 15N], 1.0 mM; Ca2+/CaM-dependent (rat) protein kinase I 1.2 mM

Ex-cond_1: pH: 6.5; temperature: 303 K; ionic strength: 0.16 M

Experiments:

NameSampleSample stateSample conditions
1H-15N-TROSY-HSQCnot availablenot availablenot available

Software:

felix v97.0 -

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAC40950 BAE22345 BAG37428 BAG70091 BAG70221 BAE22345 BAG37428 BAC40950
GB AAA19670 AAA66944 AAA99458 AAH14825 AAH71177
PRF 2024225A 2024225A
REF NP_001070336 NP_001177124 NP_001244800 NP_003647 NP_598687 XP_001095449 NP_598687 NP_604463 NP_001070336 NP_003647
SP Q14012 Q63450 Q91YS8
TPG DAA17160
SWISS-PROT Q63450 Q91YS8 Q14012
GenBank AAH71177 AAA99458 AAH14825 AAA19670 AAA66944

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts