BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5584

Title: 13C Chemical Shift Assignments of Cu(II) plastocyanin from Synechocystis sp. PCC6803   PubMed: 12581200

Deposition date: 2002-11-07 Original release date: 2003-04-10

Authors: Gelis, Ioannis; Katsaros, Nikolaos; Luchinat, Claudio; Piccioli, Mario; Poggi, Luisa

Citation: Gelis, Ioannis; Katsaros, Nikolaos; Luchinat, Claudio; Piccioli, Mario; Poggi, Luisa. "A Simple Protocol to study Blue Copper Proteins by NMR"  Eur. J. Biochem. 270, 600-609 (2003).

Assembly members:
Plastocyanin, polymer, 98 residues, Formula weight is not available
CU, non-polymer, 63.546 Da.

Natural source:   Common Name: not available   Taxonomy ID: 1148   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Synechocystis sp. PCC 6803

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Plastocyanin: ANATVKMGSDSGALVFEPST VTIKAGEEVKWVNNKLSPHN IVFAADGVDADTAAKLSHKG LAFAAGESFTSTFTEPGTYT YYCEPHRGAGMVGKVVVD

Data sets:
Data typeCount
13C chemical shifts224

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1plastocyanin monomer1
2copper ion2

Entities:

Entity 1, plastocyanin monomer 98 residues - Formula weight is not available

1   ALAASNALATHRVALLYSMETGLYSERASP
2   SERGLYALALEUVALPHEGLUPROSERTHR
3   VALTHRILELYSALAGLYGLUGLUVALLYS
4   TRPVALASNASNLYSLEUSERPROHISASN
5   ILEVALPHEALAALAASPGLYVALASPALA
6   ASPTHRALAALALYSLEUSERHISLYSGLY
7   LEUALAPHEALAALAGLYGLUSERPHETHR
8   SERTHRPHETHRGLUPROGLYTHRTYRTHR
9   TYRTYRCYSGLUPROHISARGGLYALAGLY
10   METVALGLYLYSVALVALVALASP

Entity 2, copper ion - Cu - 63.546 Da.

1   CU

Samples:

sample_1: Plastocyanin, [U-13C; U-15N], 2.0 mM

Ex-cond_1: pH: 5.2 na; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableEx-cond_1
1H-13C HSQCsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
CBCANHsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1

Software:

Sparky v3 - spectra assignment

Xwinnmr v3.1 - data processing

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 5475
PDB
DBJ BAA10227 BAK51011 BAL30009 BAL33178 BAL36347
EMBL CAA38038
GB AGF52520
REF NP_442157 WP_010873453 YP_005384024 YP_005387193 YP_005409900
SP P21697