BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5830

Title: Backbone and sidechain heteronuclear resonance assignments and hyperfine nuclear magnetic resonance shifts in horse cytochrome c   PubMed: 12931009

Deposition date: 2003-06-12 Original release date: 2003-09-28

Authors: Liu, Weixia; Rumbley, Jon; Englander, S.; Wand, A.

Citation: Liu, Weixia; Rumbley, Jon; Englander, S.; Wand, A.. "Backbone and Side-chain Heteronuclear Resonance Assignments and Hyperfine NMR Shifts in Horse Cytochrome c "  Protein Sci. 12, 2104-2108 (2003).

Assembly members:
Horse cytochrome c, polymer, 104 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: 9796   Taxonomy ID: Horse   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Horse cytochrome c: GDVEKGKKIFVQKCAQCHTV EKGGKHKTGPNLHGLFGRKT GQAPGFTYTDANKNKGITWK EETLMEYLENPKKYIPGTKM IFAGIKKKTEREDLIAYLKK ATNE

Data sets:
Data typeCount
1H chemical shifts98
15N chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c1
2heme2

Entities:

Entity 1, cytochrome c 104 residues - Formula weight is not available

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   VALGLNLYSCYSALAGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYPHETHRTYRTHRASP
6   ALAASNLYSASNLYSGLYILETHRTRPLYS
7   GLUGLUTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEALAGLYILELYSLYSLYSTHRGLU
10   ARGGLUASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: Horse cytochrome c 8.0 mM; sodium phosphate 50 mM; H2O 95%; D20 5%

sample_cond_1: pH: 5.8; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

SWISS-PROT P68097 P68096 P00004
REF XP_001498872
PRF 610169A
GenBank AAB33495
PDB
BMRB 948 947 946 944 673 672 665 645 630 5829 5828 5827 5660 546 545 544 543 5372 5026 499 4810 4809 4808 4805 439 438 437 436 4189 336 317 316 286 285 274 244 243 2368 2367 2366 224 220 216 1789 1787 1785 1783 1736 1404 1171 1170 1116 1114 1113 1112 1111 1110 1109 1108 1107 1058

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts