BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6659

Title: 15N hyperfine shifts assignment for wild-type rubredoxin from Clostridium pasteurianum in the oxidized state

Deposition date: 2005-06-08 Original release date: 2005-12-14

Authors: Lin, I-Jin; Gebel, Erika; Machonkin, Timothy; Westler, William; Markley, John

Citation: Lin, I-Jin; Gebel, Erika; Machonkin, Timothy; Westler, William; Markley, John. "Changes in hydrogen-bond strengths explain reduction potentials in 10 rubredoxin variants."  Proc. Natl. Acad. Sci. U. S. A. 11, 14581-14586 (2005).

Assembly members:
rubredoxin, polymer, 54 residues, Formula weight is not available
FE, non-polymer, 55.845 Da.

Natural source:   Common Name: Clostridium pasteurianum   Taxonomy ID: 1501   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium pasteurianum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
rubredoxin: MKKYTCTVCGYIYNPEDGDP DNGVNPGTDFKDIPDDWVCP LCGVGKDQFEEVEE

Data sets:
Data typeCount
15N chemical shifts12

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rubredoxin1
2FE (III) ION2

Entities:

Entity 1, rubredoxin 54 residues - Formula weight is not available

1   METLYSLYSTYRTHRCYSTHRVALCYSGLY
2   TYRILETYRASNPROGLUASPGLYASPPRO
3   ASPASNGLYVALASNPROGLYTHRASPPHE
4   LYSASPILEPROASPASPTRPVALCYSPRO
5   LEUCYSGLYVALGLYLYSASPGLNPHEGLU
6   GLUVALGLUGLU

Entity 2, FE (III) ION - Fe - 55.845 Da.

1   FE

Samples:

Wild-type_oxidized_rubredoxin: rubredoxin mM; phospate buffer 50 mM

50mM_phospate_buffer: pH: 6; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1D 15NWild-type_oxidized_rubredoxinisotropic50mM_phospate_buffer

Software:

No software information available

NMR spectrometers:

  • Bruker . 500 MHz

Related Database Links:

BMRB 4050 4051 4066 4137 4182 4319 4320 5600 6660 6661 6662 6663 6664 6667 6668 6669 6670 6673 6674 6675 6676 6677 6678
PDB
GB AAA23279 AJA49845 AJA53833 ELP57804 KER11884
REF WP_003447684
SP P00268