BMRB Entry 7280
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7280
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Title: 1H, 13C, and 15N chemical shift assignments for non-aromatic parts of GB1 domain PubMed: 18076052
Deposition date: 2006-08-29 Original release date: 2008-02-04
Authors: Tunnicliffe, Richard; Williamson, Mike
Citation: Wilton, David; Tunnicliffe, Richard; Kamatari, Yuji; Akasaka, Kazuyuki; Williamson, Mike. "Pressure-Induced Changes in the Solution Structure of the GB1 Domain of Protein G" Proteins 71, 1432-1440 (2008).
Assembly members:
GB1, polymer, 58 residues, Formula weight is not available
Natural source: Common Name: Streptococcus Taxonomy ID: 1301 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptococcus not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
GB1: AMDTYKLILNGKTLKGETTT
EAVDAATAEKVFKQYANDNG
VDGEWTYDDATKTFTVTE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 167 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 364 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GB1 domain | 1 |
Entities:
Entity 1, GB1 domain 58 residues - Formula weight is not available
| 1 | ALA | MET | ASP | THR | TYR | LYS | LEU | ILE | LEU | ASN | ||||
| 2 | GLY | LYS | THR | LEU | LYS | GLY | GLU | THR | THR | THR | ||||
| 3 | GLU | ALA | VAL | ASP | ALA | ALA | THR | ALA | GLU | LYS | ||||
| 4 | VAL | PHE | LYS | GLN | TYR | ALA | ASN | ASP | ASN | GLY | ||||
| 5 | VAL | ASP | GLY | GLU | TRP | THR | TYR | ASP | ASP | ALA | ||||
| 6 | THR | LYS | THR | PHE | THR | VAL | THR | GLU |
Samples:
sample_1: GB1, [U-13C; U-15N], 2 ± 0.4 mM; sodium acetate, [U-2H], 100 ± 5 mM
conditions_1: pH: 5.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| triple resonance | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| BMRB | 15156 15380 16053 16444 16627 16670 16755 16873 16882 16958 17810 18397 19394 5569 6280 6988 |
| PDB | |
| EMBL | CAA37410 |
| GB | AAY41168 |
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