BMRB Entry 19649
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19649
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Title: CD79a cytosolic domain phosphorylated, denatured state PubMed: 23667548
Deposition date: 2013-12-03 Original release date: 2014-02-11
Authors: Rosenlow, Joakim
Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Rosenlow, Joakim; Brutscher, Bernhard; Karlsson, B. Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains." PLoS ONE 8, e62947-e62947 (2013).
Assembly members:
CD79a_cyt_phosph_assigned_chem_shift_list, polymer, 63 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
CD79a_cyt_phosph_assigned_chem_shift_list: SLRKRWQNEKLGLDAGDEYE
DENLYEGLNLDDCSMYEDIS
RGLQGTYQDVGSLNIGDVQL
EKP
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 173 |
15N chemical shifts | 60 |
1H chemical shifts | 60 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | CD79a_cyt_phosp | 1 |
Entities:
Entity 1, CD79a_cyt_phosp 63 residues - Formula weight is not available
1 | SER | LEU | ARG | LYS | ARG | TRP | GLN | ASN | GLU | LYS | ||||
2 | LEU | GLY | LEU | ASP | ALA | GLY | ASP | GLU | TYR | GLU | ||||
3 | ASP | GLU | ASN | LEU | TYR | GLU | GLY | LEU | ASN | LEU | ||||
4 | ASP | ASP | CYS | SER | MET | TYR | GLU | ASP | ILE | SER | ||||
5 | ARG | GLY | LEU | GLN | GLY | THR | TYR | GLN | ASP | VAL | ||||
6 | GLY | SER | LEU | ASN | ILE | GLY | ASP | VAL | GLN | LEU | ||||
7 | GLU | LYS | PRO |
Samples:
CD79a_cytosolic_domain_phosphorylated_denatured_state: CD79a_cyt_phosp, [U-99% 13C; U-99% 15N], 200-300 uM; DTT 2 mM; sodium phosphate 20 mM; sodium azide 0.02%; Complete EDTA-free 1 X; TSP 100 uM; magnesium chloride 12 mM; ATP 1 mM; Fyn kinase 300 nM; sodium chloride 150 mM; urea 6 M; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.000; pressure: 1.000 atm; temperature: 288.000 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | CD79a_cytosolic_domain_phosphorylated_denatured_state | isotropic | sample_conditions_1 |
3D HN(ca)CO | CD79a_cytosolic_domain_phosphorylated_denatured_state | isotropic | sample_conditions_1 |
3D HNCA | CD79a_cytosolic_domain_phosphorylated_denatured_state | isotropic | sample_conditions_1 |
3D HNCACB | CD79a_cytosolic_domain_phosphorylated_denatured_state | isotropic | sample_conditions_1 |
3D HNCO | CD79a_cytosolic_domain_phosphorylated_denatured_state | isotropic | sample_conditions_1 |
3D HN(CO)CA | CD79a_cytosolic_domain_phosphorylated_denatured_state | isotropic | sample_conditions_1 |
3D HN(COCA)CB | CD79a_cytosolic_domain_phosphorylated_denatured_state | isotropic | sample_conditions_1 |
Software:
AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment
ANALYSIS v2.2, CCPN - Spectrum analysis
MDDNMR v2.1, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectrum processing
TANSY v1.0, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectra processing & analysis
NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing
NMR spectrometers:
- Varian UnityInova 600 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts