BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26957

Title: ICP4 DNA binding domain   PubMed: 28505309

Deposition date: 2016-11-24 Original release date: 2017-05-23

Authors: Tunnicliffe, Richard; Golovanov, Alexander

Citation: Tunnicliffe, Richard; Lockhart-Cairns, Michael; Levy, Colin; Mould, A Paul; Jowitt, Thomas; Sito, Hilary; Baldock, Clair; Sandri-Goldin, Rozanne; Golovanov, Alexander. "The herpes viral transcription factor ICP4 forms a novel DNA recognition complex"  Nucleic Acids Res. 45, 8064-8078 (2017).

Assembly members:
ICP4_DNA_binding_domain, polymer, 231 residues, 24367.4 Da.

Natural source:   Common Name: Human herpesvirus 1   Taxonomy ID: 10298   Superkingdom: Viruses   Kingdom: not available   Genus/species: Simplexvirus herpesvirus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ICP4_DNA_binding_domain: GPAASAGRIERRRARAAVAG RDATGRFTAGQPRRVELDAD ATSGAFYARYRDGYVSGEPW PGAGPPPPGRVLYGGLGDSR PGLWGAPEAEEARRRFEASG APAAVWAPELGDAAQQYALI TRLLYTPDAEAMGWLQNPRV VPGDVALDQACFRISGAARN SSSFITGSVARAVPHLGYAM AAGRFGWGLAHAAAAVAMSR RYDRAQKGFLLTSLRRAYAP LLARENAALTG

Data sets:
Data typeCount
15N chemical shifts34
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ICP4N1

Entities:

Entity 1, ICP4N 231 residues - 24367.4 Da.

N-terminal glycine is non-native. Remains after HRV3C protease cleavage.

1   GLYPROALAALASERALAGLYARGILEGLU
2   ARGARGARGALAARGALAALAVALALAGLY
3   ARGASPALATHRGLYARGPHETHRALAGLY
4   GLNPROARGARGVALGLULEUASPALAASP
5   ALATHRSERGLYALAPHETYRALAARGTYR
6   ARGASPGLYTYRVALSERGLYGLUPROTRP
7   PROGLYALAGLYPROPROPROPROGLYARG
8   VALLEUTYRGLYGLYLEUGLYASPSERARG
9   PROGLYLEUTRPGLYALAPROGLUALAGLU
10   GLUALAARGARGARGPHEGLUALASERGLY
11   ALAPROALAALAVALTRPALAPROGLULEU
12   GLYASPALAALAGLNGLNTYRALALEUILE
13   THRARGLEULEUTYRTHRPROASPALAGLU
14   ALAMETGLYTRPLEUGLNASNPROARGVAL
15   VALPROGLYASPVALALALEUASPGLNALA
16   CYSPHEARGILESERGLYALAALAARGASN
17   SERSERSERPHEILETHRGLYSERVALALA
18   ARGALAVALPROHISLEUGLYTYRALAMET
19   ALAALAGLYARGPHEGLYTRPGLYLEUALA
20   HISALAALAALAALAVALALAMETSERARG
21   ARGTYRASPARGALAGLNLYSGLYPHELEU
22   LEUTHRSERLEUARGARGALATYRALAPRO
23   LEULEUALAARGGLUASNALAALALEUTHR
24   GLY

Samples:

sample_1: ICP4 DNA binding domain, [U-100% 15N], 0.3 ± 0.01 mM; MES 20 mM; NaCl 50 mM; L-Arg 50 mM; L-Glu 50 mM; TCEP 1 mM; MgCl2 2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY v3.114, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 800 MHz

Related Database Links:

EMBL CAA32286
Uniprot P08392

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts