BMRB Entry 10096
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10096
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Title: Backbone Resonance Assignments for LolB PubMed: 19636844
Deposition date: 2007-01-31 Original release date: 2007-08-22
Authors: Nakada, Shingo; Sakakura, Masayoshi; Takahashi, Hideo; Tokuda, Hajime; Shimada, Ichio
Citation: Shingo, Nakada; Masayoshi, Sakakura; Hideo, Takahashi; Hajime, Tokuda; Ichio, Shimada. "Backbone resonance assignment for the outer membrane lipoprotein receptor LolB from Escherichia coli" Biomol. NMR Assignments 1, 121-123 (2007).
Assembly members:
LolB, polymer, 187 residues, 21300 Da.
Natural source: Common Name: Escherichia coli K-12 Taxonomy ID: 83333 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
LolB: MASVTTPKGPGKSPDSPQWR
QHQQDVRNLNQYQTRGAFAY
ISDQQKVYARFFWQQTGQDR
YRLLLTNPLGSTELELNAQP
GNVQLVDNKGQRYTADDAEE
MIGKLTGMPIPLNSLRQWIL
GLPGDATDYKLDDQYRLSEI
TYSQNGKNWKVVYGGYDTKT
QPAMPANMELTDGGQRIKLK
MDNWIVK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 476 |
| 15N chemical shifts | 159 |
| 1H chemical shifts | 159 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LolB monomer | 1 |
Entities:
Entity 1, LolB monomer 187 residues - 21300 Da.
| 1 | MET | ALA | SER | VAL | THR | THR | PRO | LYS | GLY | PRO | ||||
| 2 | GLY | LYS | SER | PRO | ASP | SER | PRO | GLN | TRP | ARG | ||||
| 3 | GLN | HIS | GLN | GLN | ASP | VAL | ARG | ASN | LEU | ASN | ||||
| 4 | GLN | TYR | GLN | THR | ARG | GLY | ALA | PHE | ALA | TYR | ||||
| 5 | ILE | SER | ASP | GLN | GLN | LYS | VAL | TYR | ALA | ARG | ||||
| 6 | PHE | PHE | TRP | GLN | GLN | THR | GLY | GLN | ASP | ARG | ||||
| 7 | TYR | ARG | LEU | LEU | LEU | THR | ASN | PRO | LEU | GLY | ||||
| 8 | SER | THR | GLU | LEU | GLU | LEU | ASN | ALA | GLN | PRO | ||||
| 9 | GLY | ASN | VAL | GLN | LEU | VAL | ASP | ASN | LYS | GLY | ||||
| 10 | GLN | ARG | TYR | THR | ALA | ASP | ASP | ALA | GLU | GLU | ||||
| 11 | MET | ILE | GLY | LYS | LEU | THR | GLY | MET | PRO | ILE | ||||
| 12 | PRO | LEU | ASN | SER | LEU | ARG | GLN | TRP | ILE | LEU | ||||
| 13 | GLY | LEU | PRO | GLY | ASP | ALA | THR | ASP | TYR | LYS | ||||
| 14 | LEU | ASP | ASP | GLN | TYR | ARG | LEU | SER | GLU | ILE | ||||
| 15 | THR | TYR | SER | GLN | ASN | GLY | LYS | ASN | TRP | LYS | ||||
| 16 | VAL | VAL | TYR | GLY | GLY | TYR | ASP | THR | LYS | THR | ||||
| 17 | GLN | PRO | ALA | MET | PRO | ALA | ASN | MET | GLU | LEU | ||||
| 18 | THR | ASP | GLY | GLY | GLN | ARG | ILE | LYS | LEU | LYS | ||||
| 19 | MET | ASP | ASN | TRP | ILE | VAL | LYS |
Samples:
sample_1: LolB, [U-2H; U-13C; U-15N], 0.5 – 1.0 mM; MES 38 mM; D2O 10%; H2O 90%
condition_1: pH: 6.9; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N TROSY-HSQC | sample_1 | not available | condition_1 |
| TROSY-HNCACB | sample_1 | not available | condition_1 |
| TROSY-HN(CO)CACB | sample_1 | not available | condition_1 |
| TROSY-HNCA | sample_1 | not available | condition_1 |
| TROSY-HN(CO)CA | sample_1 | not available | condition_1 |
| TROSY-HNCO | sample_1 | not available | condition_1 |
Software:
xwinnmr v3.5, Bruker Japan - data collection
SPARKY v3.110, T. D. Goddard and D. G. Kneller - peak assignments
NMRPipe v2.3 - data processing
NMR spectrometers:
- Bruker Avance600 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA01105 BAA36067 BAB35137 BAG76783 BAI25021 |
| EMBL | CAP75748 CAQ31711 CAQ89267 CAQ98089 CAR02603 |
| GB | AAA24433 AAC74293 AAG56067 AAN42825 AAN80132 |
| REF | NP_309741 NP_415727 NP_707118 WP_000174480 WP_001130605 |
| SP | A7ZKY3 A7ZZE5 B1IU84 B1LH91 B1XAQ0 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts