BMRB Entry 15036
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15036
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Title: SOLUTION STRUCTURE OF HUMAN BETA-MICROSEMINOPROTEIN PubMed: 16930619
Deposition date: 2006-11-17 Original release date: 2007-01-10
Authors: Ghasriani, H.; Teilum, K.; Johnsson, Y.; Fernlund, P.; Drakenberg, T.
Citation: Ghasriani, Houman; Teilum, Kaare; Johnsson, Ylva; Fernlund, Per; Drakenberg, Torbjorn. "Solution structure of human and porcine beta-microseminoprotein." J. Mol. Biol. 362, 502-515 (2006).
Assembly members:
hMSP, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
hMSP: GGGSCYFIPNEGVPGDSTRK
CMDLKGNKHPINSEWQTDNC
ETCTCYETEISCCTLVSTPV
GYDKDNCQRIFKKEDCKYIV
VEKKDPKKTCSVSEWII
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 354 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 591 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hMSP | 1 |
Entities:
Entity 1, hMSP 97 residues - Formula weight is not available
| 1 | GLY | GLY | GLY | SER | CYS | TYR | PHE | ILE | PRO | ASN | ||||
| 2 | GLU | GLY | VAL | PRO | GLY | ASP | SER | THR | ARG | LYS | ||||
| 3 | CYS | MET | ASP | LEU | LYS | GLY | ASN | LYS | HIS | PRO | ||||
| 4 | ILE | ASN | SER | GLU | TRP | GLN | THR | ASP | ASN | CYS | ||||
| 5 | GLU | THR | CYS | THR | CYS | TYR | GLU | THR | GLU | ILE | ||||
| 6 | SER | CYS | CYS | THR | LEU | VAL | SER | THR | PRO | VAL | ||||
| 7 | GLY | TYR | ASP | LYS | ASP | ASN | CYS | GLN | ARG | ILE | ||||
| 8 | PHE | LYS | LYS | GLU | ASP | CYS | LYS | TYR | ILE | VAL | ||||
| 9 | VAL | GLU | LYS | LYS | ASP | PRO | LYS | LYS | THR | CYS | ||||
| 10 | SER | VAL | SER | GLU | TRP | ILE | ILE |
Samples:
sample_1: entity, [U-95% 13C; U-95% 15N], 3.4 – 4.2 mM; H20 90%; D20 10%
sample_2: entity, [U-95% 13C; U-95% 15N], 3.4 – 4.2 mM; D20 100%
sample_conditions_1: ionic strength: 1 mM; pH: 6.0; pressure: 1 atm; temperature: 310.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, CLORE - refinement
NMR spectrometers:
- VARIAN UNITYINOVA 600 MHz
Related Database Links:
| BMRB | 15037 |
| PDB | |
| EMBL | CAA41002 CAB39325 CAG33296 |
| GB | AAA36635 AAA59871 AAB29732 AAB37355 AAH05257 |
| PRF | 1209281A |
| REF | NP_002434 |
| SP | P08118 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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