BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15110

Title: Solution structure of V21C/V59C Lymphotactin/XCL1   PubMed: 17302442

Deposition date: 2007-01-23 Original release date: 2007-05-04

Authors: Volkman, B.; Tuinstra, R.; Peterson, F.; Elgin, E.

Citation: Tuinstra, R.; Peterson, F.; Elgin, E.; Pelzek, A.; Volkman, B.. "An engineered second disulfide bond restricts lymphotactin/XCL1 to a chemokine-like conformation with XCR1 agonist activity"  Biochemistry 46, 2564-2573 (2007).

Assembly members:
Lymphotactin, polymer, 92 residues, 10198.776 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Lymphotactin: GSEVSDKRTCVSLTTQRLPC SRIKTYTITEGSLRAVIFIT KRGLKVCADPQATWVRDCVR SMDRKSNTRNNMIQTKPTGT QQSTNTAVTLTG

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts88
1H chemical shifts593

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lymphotactin1

Entities:

Entity 1, Lymphotactin 92 residues - 10198.776 Da.

1   GLYSERGLUVALSERASPLYSARGTHRCYS
2   VALSERLEUTHRTHRGLNARGLEUPROCYS
3   SERARGILELYSTHRTYRTHRILETHRGLU
4   GLYSERLEUARGALAVALILEPHEILETHR
5   LYSARGGLYLEULYSVALCYSALAASPPRO
6   GLNALATHRTRPVALARGASPCYSVALARG
7   SERMETASPARGLYSSERASNTHRARGASN
8   ASNMETILEGLNTHRLYSPROTHRGLYTHR
9   GLNGLNSERTHRASNTHRALAVALTHRLEU
10   THRGLY

Samples:

sample: Lymphotactin, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sampleisotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

NMRPipe v2004, Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A. - processing

XEASY v1.3, Bartels, C, Xia, T.-H., Billeter, M., Guntert, P., Wuthrich, K. - data analysis

GARANT v2.1, Bartels, C., Billeter, M., Guntert, P., Wuthrich, K. - data analysis

CYANA v2.1, Herrmann, T., Guntert, P., Wuthrich, K. - structure solution

X-PLOR NIH v2.9.3, Schwieters, C.D., Kuszewski, J.J, Tjandra, N., Clore, G.M. - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15215
PDB
DBJ BAA07825 BAA09859
EMBL CAA60198
GB AAC50164 AAH69817 AAH70309 AIC49701 AJA36131
REF NP_002986 XP_003824662 XP_004027907 XP_009436138 XP_011508167
SP P47992

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts