BMRB Entry 15326
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15326
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Title: Backbone 13C, HN and 15N assignments for human chemerin PubMed: 19636857
Deposition date: 2007-06-22 Original release date: 2007-09-17
Authors: Allen, Samantha; Handel, Tracy; Zabel, Brian; Nietlispach, Daniel; Butcher, Eugene; Kirkpatrick, John
Citation: Allen, Samantha; Zabel, Brian; Kirkpatrick, John; Butcher, Eugene; Nietlispach, Daniel; Handel, Tracy. "NMR assignment of human chemerin, a novel chemoattractant" Biomol. NMR Assignments 1, 171-173 (2007).
Assembly members:
Chemerin, polymer, 145 residues, 16962 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Chemerin: MELTEAQRRGLQVALEEFHK
HPPVQWAFQETSVESAVDTP
FPAGIFVRLEFKLQQTSCRK
RDWKKPECKVRPNGRKRKCL
ACIKLGSEDKVLGRLVHCPI
ETQVLREAEEHQETQCLRVQ
RAGEDPHSFYFPGQFAPHHH
HHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 498 |
| 15N chemical shifts | 122 |
| 1H chemical shifts | 122 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chemerin | 1 |
Entities:
Entity 1, chemerin 145 residues - 16962 Da.
Residue 1 is a non-native initiator met. The last 9 residues are also non-native (a proline and His8 tag).
| 1 | MET | GLU | LEU | THR | GLU | ALA | GLN | ARG | ARG | GLY | ||||
| 2 | LEU | GLN | VAL | ALA | LEU | GLU | GLU | PHE | HIS | LYS | ||||
| 3 | HIS | PRO | PRO | VAL | GLN | TRP | ALA | PHE | GLN | GLU | ||||
| 4 | THR | SER | VAL | GLU | SER | ALA | VAL | ASP | THR | PRO | ||||
| 5 | PHE | PRO | ALA | GLY | ILE | PHE | VAL | ARG | LEU | GLU | ||||
| 6 | PHE | LYS | LEU | GLN | GLN | THR | SER | CYS | ARG | LYS | ||||
| 7 | ARG | ASP | TRP | LYS | LYS | PRO | GLU | CYS | LYS | VAL | ||||
| 8 | ARG | PRO | ASN | GLY | ARG | LYS | ARG | LYS | CYS | LEU | ||||
| 9 | ALA | CYS | ILE | LYS | LEU | GLY | SER | GLU | ASP | LYS | ||||
| 10 | VAL | LEU | GLY | ARG | LEU | VAL | HIS | CYS | PRO | ILE | ||||
| 11 | GLU | THR | GLN | VAL | LEU | ARG | GLU | ALA | GLU | GLU | ||||
| 12 | HIS | GLN | GLU | THR | GLN | CYS | LEU | ARG | VAL | GLN | ||||
| 13 | ARG | ALA | GLY | GLU | ASP | PRO | HIS | SER | PHE | TYR | ||||
| 14 | PHE | PRO | GLY | GLN | PHE | ALA | PRO | HIS | HIS | HIS | ||||
| 15 | HIS | HIS | HIS | HIS | HIS |
Samples:
13C_15N_chemerin: Chemerin, [U-98% 13C; U-98% 15N], 0.8 – 1.5 mM; acetic acid, [U-2H], 10 mM; EDTA, [U-2H], 0.1 mM
15N_chemerin: Chemerin, [U-98% 15N], 0.8 mM; EDTA, [U-2H], 0.1 mM; acetic acid, [U-2H], 10 mM
sample_conditions_1: pH: 5.49; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | 15N_chemerin | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | 13C_15N_chemerin | isotropic | sample_conditions_1 |
| 3D C(CO)NH | 13C_15N_chemerin | isotropic | sample_conditions_1 |
| 3D HNCO | 13C_15N_chemerin | isotropic | sample_conditions_1 |
| CBCANH | 13C_15N_chemerin | isotropic | sample_conditions_1 |
Software:
AZARA v2.7, Boucher - processing
Analysis v1.0.14, CCPN - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| DBJ | BAA76499 BAG35130 |
| EMBL | CAG46789 CAG46823 CAH93115 |
| GB | AAB47975 AAH00069 AAS00384 AAX36662 AAX37088 |
| REF | NP_001127631 NP_002880 XP_001134678 XP_003815095 XP_004046507 |
| SP | Q5R551 Q99969 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts