BMRB Entry 15892
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15892
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Title: Backbone 1HN and 15N Assignments for Native Human alpha-Lactalbumin PubMed: 19309110
Deposition date: 2008-07-25 Original release date: 2009-04-22
Authors: Redfield, Christina
Citation: Bruylants, Gilles; Redfield, Christina. "(15)N NMR relaxation data reveals significant chemical exchange broadening in the alpha-domain of human alpha-lactalbumin" Biochemistry 48, 4031-4039 (2009).
Assembly members:
alpha-lactalbumin, polymer, 124 residues, Formula weight is not available
CA_2+, non-polymer, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
alpha-lactalbumin: MKQFTKCELSQLLKDIDGYG
GIALPELICTMFHTSGYDTQ
AIVENNESTEYGLFQISNKL
WCKSSQVPQSRNICDISCDK
FLDDDITDDIMCAKKILDIK
GIDYWLAHKALCTEKLEQWL
CEKL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 115 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | alpha-lactalbumin | 1 |
| 2 | CA_2+ | 2 |
Entities:
Entity 1, alpha-lactalbumin 124 residues - Formula weight is not available
The N-terminal methionine is called MET-0
| 1 | MET | LYS | GLN | PHE | THR | LYS | CYS | GLU | LEU | SER | ||||
| 2 | GLN | LEU | LEU | LYS | ASP | ILE | ASP | GLY | TYR | GLY | ||||
| 3 | GLY | ILE | ALA | LEU | PRO | GLU | LEU | ILE | CYS | THR | ||||
| 4 | MET | PHE | HIS | THR | SER | GLY | TYR | ASP | THR | GLN | ||||
| 5 | ALA | ILE | VAL | GLU | ASN | ASN | GLU | SER | THR | GLU | ||||
| 6 | TYR | GLY | LEU | PHE | GLN | ILE | SER | ASN | LYS | LEU | ||||
| 7 | TRP | CYS | LYS | SER | SER | GLN | VAL | PRO | GLN | SER | ||||
| 8 | ARG | ASN | ILE | CYS | ASP | ILE | SER | CYS | ASP | LYS | ||||
| 9 | PHE | LEU | ASP | ASP | ASP | ILE | THR | ASP | ASP | ILE | ||||
| 10 | MET | CYS | ALA | LYS | LYS | ILE | LEU | ASP | ILE | LYS | ||||
| 11 | GLY | ILE | ASP | TYR | TRP | LEU | ALA | HIS | LYS | ALA | ||||
| 12 | LEU | CYS | THR | GLU | LYS | LEU | GLU | GLN | TRP | LEU | ||||
| 13 | CYS | GLU | LYS | LEU |
Entity 2, CA_2+ 1 residues - CA1 - Formula weight is not available
| 1 | CA_2+ |
Samples:
sample_1: alpha-lactalbumin, [U-99% 15N], 1 mM; CA_2+ 3 mM; H2O 95%; D2O, [U-100% 2H], 5%
sample_conditions_1: ionic strength: 0 M; pH: 6.3; pressure: 1 atm; temperature: 291 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX v2.3, Accelrys Software Inc. - processing
NMR spectrometers:
- GE OMEGA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAC06860 BAJ20953 |
| EMBL | CAA28799 CAA28800 CAG46784 CAG46814 |
| GB | AAA60345 AAH69103 AAI12317 AAI12319 AAX36575 |
| REF | NP_002280 XP_001102116 XP_001160401 XP_002823206 XP_003252260 |
| SP | P00709 |
| TPE | CDM98741 CDM98755 CDM98761 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts