BMRB Entry 16044
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16044
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Title: N-terminal backbone assignment of the polyoma virus large T antigen PubMed: 19636961
Deposition date: 2008-11-27 Original release date: 2009-04-16
Authors: Knoblich, Konstantin; Jiang, Tao; Schaffhausen, Brian; Gunther, Ulrich
Citation: Knoblich, Konstantin; Whittaker, Sara; Ludwig, Christian; Michiels, Paul; Jiang, Tao; Schaffhausen, Brian; Gunther, Ulrich. "Backbone assignment of the N-terminal polyomavirus large T antigen" Biomol. NMR Assignments 3, 119-123 (2009).
Assembly members:
PyLTNT, polymer, 264 residues, Formula weight is not available
Natural source: Common Name: Murine polyomavirus Taxonomy ID: 10634 Superkingdom: Viruses Kingdom: not available Genus/species: Polyomavirus Murine polyomavirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PyLTNT: MDRVLSRADKERLLELLKLP
RQLWGDFGRMQQAYKQQSLL
LHPDKGGSHALMQELNSLWG
TFKTEVYNLRMNLGGTGFQG
SPPRTAERGTEESGHSPLHD
DYWSFSYGSKYFTREWNDFF
RKWDPSYQSPPKTAESSEQP
DLFCYEEPLLSPNPSSPTDT
PAHTAGRRRNPCVAEPDDSI
SPDPPRTPVSRKRPRPAGAT
GGGGGGVHANGGSVFGHPTG
GTSTPAHPPPYHSQGGSESM
GGSDSSGFAEGSFRSDGSHH
HHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 406 |
| 15N chemical shifts | 142 |
| 1H chemical shifts | 261 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PyLTNT | 1 |
Entities:
Entity 1, PyLTNT 264 residues - Formula weight is not available
| 1 | MET | ASP | ARG | VAL | LEU | SER | ARG | ALA | ASP | LYS | ||||
| 2 | GLU | ARG | LEU | LEU | GLU | LEU | LEU | LYS | LEU | PRO | ||||
| 3 | ARG | GLN | LEU | TRP | GLY | ASP | PHE | GLY | ARG | MET | ||||
| 4 | GLN | GLN | ALA | TYR | LYS | GLN | GLN | SER | LEU | LEU | ||||
| 5 | LEU | HIS | PRO | ASP | LYS | GLY | GLY | SER | HIS | ALA | ||||
| 6 | LEU | MET | GLN | GLU | LEU | ASN | SER | LEU | TRP | GLY | ||||
| 7 | THR | PHE | LYS | THR | GLU | VAL | TYR | ASN | LEU | ARG | ||||
| 8 | MET | ASN | LEU | GLY | GLY | THR | GLY | PHE | GLN | GLY | ||||
| 9 | SER | PRO | PRO | ARG | THR | ALA | GLU | ARG | GLY | THR | ||||
| 10 | GLU | GLU | SER | GLY | HIS | SER | PRO | LEU | HIS | ASP | ||||
| 11 | ASP | TYR | TRP | SER | PHE | SER | TYR | GLY | SER | LYS | ||||
| 12 | TYR | PHE | THR | ARG | GLU | TRP | ASN | ASP | PHE | PHE | ||||
| 13 | ARG | LYS | TRP | ASP | PRO | SER | TYR | GLN | SER | PRO | ||||
| 14 | PRO | LYS | THR | ALA | GLU | SER | SER | GLU | GLN | PRO | ||||
| 15 | ASP | LEU | PHE | CYS | TYR | GLU | GLU | PRO | LEU | LEU | ||||
| 16 | SER | PRO | ASN | PRO | SER | SER | PRO | THR | ASP | THR | ||||
| 17 | PRO | ALA | HIS | THR | ALA | GLY | ARG | ARG | ARG | ASN | ||||
| 18 | PRO | CYS | VAL | ALA | GLU | PRO | ASP | ASP | SER | ILE | ||||
| 19 | SER | PRO | ASP | PRO | PRO | ARG | THR | PRO | VAL | SER | ||||
| 20 | ARG | LYS | ARG | PRO | ARG | PRO | ALA | GLY | ALA | THR | ||||
| 21 | GLY | GLY | GLY | GLY | GLY | GLY | VAL | HIS | ALA | ASN | ||||
| 22 | GLY | GLY | SER | VAL | PHE | GLY | HIS | PRO | THR | GLY | ||||
| 23 | GLY | THR | SER | THR | PRO | ALA | HIS | PRO | PRO | PRO | ||||
| 24 | TYR | HIS | SER | GLN | GLY | GLY | SER | GLU | SER | MET | ||||
| 25 | GLY | GLY | SER | ASP | SER | SER | GLY | PHE | ALA | GLU | ||||
| 26 | GLY | SER | PHE | ARG | SER | ASP | GLY | SER | HIS | HIS | ||||
| 27 | HIS | HIS | HIS | HIS |
Samples:
sample_1: PyLTNT, [U-99% 15N], 700 uM; KCl 150 mM
sample_2: PyLTNT, [U-99% 13C; U-99% 15N], 700 uM; KCl 150 mM
sample_3: PyLTNT, [U-99% 13C; U-99% 15N], 400 uM; KCl 150 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HNN | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCN | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCACON | sample_3 | isotropic | sample_conditions_1 |
| 3D CBCANCO | sample_3 | isotropic | sample_conditions_1 |
| 2D CON | sample_3 | isotropic | sample_conditions_1 |
Software:
Analysis, CCPN - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 900 MHz
- Varian INOVA 800 MHz
Related Database Links:
| EMBL | CAA42510 |
| GB | AAA46872 AAA93241 AAA93245 AAB59901 AAL35609 |
| REF | YP_009111413 |
| SP | P03073 P03074 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts