BMRB Entry 16168
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16168
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Title: STRUCTURE OF THE NA,K-ATPASE REGULATORY PROTEIN FXYD1 IN MICELLES PubMed: 17511473
Deposition date: 2009-02-11 Original release date: 2009-03-06
Authors: Teriete, P.; Franzin, C.; Choi, J.; Marassi, F.
Citation: Teriete, P.; Franzin, C.; Choi, J.; Marassi, F.. "Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles." Biochemistry 46, 6774-6783 (2007).
Assembly members:
FXYD1, polymer, 72 residues, 8396.635 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
FXYD1: ESPKEHDPFTYDYQSLQIGG
LVIAGILFILGILIVLSRRC
RCKFNQQQRTGEPDEEEGTF
RSSIRRLSTRRR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 138 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 145 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FXYD1 | 1 |
Entities:
Entity 1, FXYD1 72 residues - 8396.635 Da.
| 1 | GLU | SER | PRO | LYS | GLU | HIS | ASP | PRO | PHE | THR | ||||
| 2 | TYR | ASP | TYR | GLN | SER | LEU | GLN | ILE | GLY | GLY | ||||
| 3 | LEU | VAL | ILE | ALA | GLY | ILE | LEU | PHE | ILE | LEU | ||||
| 4 | GLY | ILE | LEU | ILE | VAL | LEU | SER | ARG | ARG | CYS | ||||
| 5 | ARG | CYS | LYS | PHE | ASN | GLN | GLN | GLN | ARG | THR | ||||
| 6 | GLY | GLU | PRO | ASP | GLU | GLU | GLU | GLY | THR | PHE | ||||
| 7 | ARG | SER | SER | ILE | ARG | ARG | LEU | SER | THR | ARG | ||||
| 8 | ARG | ARG |
Samples:
sample_1: FXYD1, [U-100% 13C; U-100% 15N], 0.5 mM; sodium citrate 20 mM; DTT 10 mM; SDS 500 mM; H2O 90%; D2O 10%
sample_2: FXYD1, [U-100% 15N], 1 mM; sodium citrate 20 mM; DTT 10 mM; SDS 500 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 60 mM; pH: 5.0; pressure: 1 atm; temperature: 313.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, C.D.SCHWIETERS,J.J.KUSZEWSKI,N.TJ - refinement
SPARKY v3.11, Goddard - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMR spectrometers:
- BRUKER DMX 600 MHz
- BRUKER DMX 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAF82500 BAG73170 |
| GB | AAC51286 AAH32800 ABW03332 ABW03689 ACT90639 |
| REF | NP_001265646 NP_001265647 NP_005022 NP_068702 XP_002829099 |
| SP | O00168 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts