BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16235

Title: Solution structure of linear kalata B1 (loop 6)   PubMed: 19491108

Deposition date: 2009-04-01 Original release date: 2010-01-12

Authors: Wang, Conan

Citation: Huang, Yen-Hua; Colgrave, Michelle; Daly, Norelle; Keleshian, Asbed; Martinac, Boris; Craik, David. "The biological activity of the prototypic cyclotide kalata b1 is modulated by the formation of multimeric pores."  J. Biol. Chem. 284, 20699-20707 (2009).

Assembly members:
loop 6, polymer, 29 residues, 2917.343 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
loop 6: GLPVCGETCVGGTCNTPGCT CSWPVCTRN

Data sets:
Data typeCount
1H chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1loop 61

Entities:

Entity 1, loop 6 29 residues - 2917.343 Da.

1   GLYLEUPROVALCYSGLYGLUTHRCYSVAL
2   GLYGLYTHRCYSASNTHRPROGLYCYSTHR
3   CYSSERTRPPROVALCYSTHRARGASN

Samples:

sample_1: entity 1 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1.0 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

NMR spectrometers:

  • Bruker ARX 500 MHz

Related Database Links:

BMRB 18506 18536 19611
PDB
GB AAL05477 ACI29319 ADJ17473
PIR A56283
SP P56254