BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16274

Title: SLBP PAPA peptide   PubMed: 22907757

Deposition date: 2009-05-04 Original release date: 2012-08-06

Authors: Thapar, Roopa

Citation: Krishnan, Nithya; Lam, Tukiet; Fritz, Andrew; Rempinski, Donald; Minderman, Kieran; Berezney, Hans; Marzluff, Ronald; Thapar, William. "The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination."  Mol. Cell. Biol. 32, 4306-4322 (2012).

Assembly members:
SLBP, polymer, 21 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
SLBP: HLRQPGIHAAKTANKFKKYS R

Data sets:
Data typeCount
15N chemical shifts17
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SLBP1

Entities:

Entity 1, SLBP 21 residues - Formula weight is not available

Phosphorylation site in the RNA binding domain of SLBP that exhibits phosphorylation-dependent prolyl isomerization

1   HISLEUARGGLNPROGLYILEHISALAALA
2   LYSTHRALAASNLYSPHELYSLYSTYRSER
3   ARG

Samples:

sample_1: SLBP PAPA peptide 5 mM; H2O 90%; D2O 10%; Tris acetate 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 700 MHz
  • Varian INOVA 600 MHz

Related Database Links:

GB NP_006518

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts