BMRB Entry 16274
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16274
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: SLBP PAPA peptide PubMed: 22907757
Deposition date: 2009-05-04 Original release date: 2012-08-06
Authors: Thapar, Roopa
Citation: Krishnan, Nithya; Lam, Tukiet; Fritz, Andrew; Rempinski, Donald; Minderman, Kieran; Berezney, Hans; Marzluff, Ronald; Thapar, William. "The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination." Mol. Cell. Biol. 32, 4306-4322 (2012).
Assembly members:
SLBP, polymer, 21 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
SLBP: HLRQPGIHAAKTANKFKKYS
R
- assigned_chemical_shifts
Data type | Count |
15N chemical shifts | 17 |
1H chemical shifts | 110 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | SLBP | 1 |
Entities:
Entity 1, SLBP 21 residues - Formula weight is not available
Phosphorylation site in the RNA binding domain of SLBP that exhibits phosphorylation-dependent prolyl isomerization
1 | HIS | LEU | ARG | GLN | PRO | GLY | ILE | HIS | ALA | ALA | ||||
2 | LYS | THR | ALA | ASN | LYS | PHE | LYS | LYS | TYR | SER | ||||
3 | ARG |
Samples:
sample_1: SLBP PAPA peptide 5 mM; H2O 90%; D2O 10%; Tris acetate 20 mM; NaCl 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 5; pressure: 1 atm; temperature: 278 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX, Accelrys Software Inc. - chemical shift assignment
NMR spectrometers:
- Varian INOVA 700 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts