BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16294

Title: 1H (NH, HA, HB, other) Chemical Shift Assignments of Peach Pru p 3, Non-Specific Lipid Transfer Protein (nsLTP) isolated from Peach fruit skin   PubMed: 20121231

Deposition date: 2009-05-08 Original release date: 2010-03-01

Authors: Wijesinha-Bettoni, Ramani; Alexeev, Yuri; Johnson, Phil; Marsh, Justin; Sancho, Ana; Mackie, Alan; Shewry, Peter; Smith, Lorna; Mills, E. N

Citation: Wijesinha-Bettoni, Ramani; Alexeev, Yuri; Johnson, Phil; Marsh, Justin; Sancho, Ana; Abdullah, Syed; Mackie, Alan; Shewry, Peter; Smith, Lorna; Mills, E. N Clare. "The structural characteristics of nonspecific lipid transfer proteins explain their resistance to gastroduodenal proteolysis."  Biochemistry 49, 2130-2139 (2010).

Assembly members:
Pru_p_3_(nsLTP), polymer, 91 residues, Formula weight is not available

Natural source:   Common Name: peach   Taxonomy ID: 3760   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Prunus persica

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Pru_p_3_(nsLTP): ITCGQVSSSLAPCIPYVRGG GAVPPACCNGIRNVNNLART TPDRQAACNCLKQLSASVPG VNPNNAAALPGKCGVSIPYK ISASTNCATVK

Data sets:
Data typeCount
1H chemical shifts332

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1non specific lipid transfer protein from peach (Pru p 3)1

Entities:

Entity 1, non specific lipid transfer protein from peach (Pru p 3) 91 residues - Formula weight is not available

1   ILETHRCYSGLYGLNVALSERSERSERLEU
2   ALAPROCYSILEPROTYRVALARGGLYGLY
3   GLYALAVALPROPROALACYSCYSASNGLY
4   ILEARGASNVALASNASNLEUALAARGTHR
5   THRPROASPARGGLNALAALACYSASNCYS
6   LEULYSGLNLEUSERALASERVALPROGLY
7   VALASNPROASNASNALAALAALALEUPRO
8   GLYLYSCYSGLYVALSERILEPROTYRLYS
9   ILESERALASERTHRASNCYSALATHRVAL
10   LYS

Samples:

sample_1: Pru p 3 (nsLTP)2.0 – 3.0 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 5.3; temperature: 310 K

sample_conditions_2: pH: 5.3; temperature: 298 K

sample_conditions_3: pH: 4; temperature: 298 K

sample_conditions_4: pH: 6.5; temperature: 293 K

sample_conditions_5: pH: 1.8; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_2
2D 1H-1H COSYsample_1isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_3
2D 1H-1H TOCSYsample_1isotropicsample_conditions_4
2D 1H-1H COSYsample_1isotropicsample_conditions_4
2D 1H-1H NOESYsample_1isotropicsample_conditions_4
2D 1H-1H TOCSYsample_1isotropicsample_conditions_5
2D 1H-1H COSYsample_1isotropicsample_conditions_5
2D 1H-1H NOESYsample_1isotropicsample_conditions_5

Software:

SPARKY vVersion 3.110, Goddard - data analysis

NMR spectrometers:

  • Home-built ?,AM 600 MHz

Related Database Links:

PDB
EMBL CAB96876
GB AAV40850 AAV64877 ACE80965 ADR66939 ADR66940
REF XP_007206159
SP P81402