BMRB Entry 16323
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16323
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Title: SLBP RBD 30-mer peptide PubMed: 22907757
Deposition date: 2009-05-28 Original release date: 2012-08-03
Authors: Thapar, Roopa
Citation: Krishnan, Nithya; Lam, Tukiet; Fritz, Andrew; Rempinski, Donald; Minderman, Kieran; Berezney, Hans; Marzluff, Ronald; Thapar, William. "The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination." Mol. Cell. Biol. 32, 4306-4322 (2012).
Assembly members:
SLBP30mer, polymer, 30 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
SLBP30mer: ETDESVLMRRQKQINYGKNT
IAYDRYIKEV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 169 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SLBP30mer | 1 |
Entities:
Entity 1, SLBP30mer 30 residues - Formula weight is not available
| 1 | GLU | THR | ASP | GLU | SER | VAL | LEU | MET | ARG | ARG | |
| 2 | GLN | LYS | GLN | ILE | ASN | TYR | GLY | LYS | ASN | THR | |
| 3 | ILE | ALA | TYR | ASP | ARG | TYR | ILE | LYS | GLU | VAL |
Samples:
sample_1: SLBP30mer1 – 7 mM; H2O 90%; D2O 10%; NaCl 50 mM
sample_conditions_1: ionic strength: 0.050 M; pH: 5; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX, Accelrys Software Inc. - processing
NMR spectrometers:
- Varian INOVA 700 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE20786 BAE22851 BAE32343 BAE36737 BAG38003 |
| EMBL | CAA94918 |
| GB | AAB97091 AAC53530 AAH14908 AAH15703 AAH29631 |
| REF | NP_001039345 NP_001231786 NP_001233401 NP_001276653 NP_001276654 |
| SP | P97440 Q14493 |
| TPG | DAA28416 |