BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16350

Title: NMR solution structure of the pheromone En-A1 from Euplotes nobilii   PubMed: 21300903

Deposition date: 2009-06-15 Original release date: 2011-04-08

Authors: Pedrini, Bill; Alimenti, Claudio; Vallesi, Adriana; Luporini, Piero; Wuthrich, Kurt

Citation: Di Giuseppe, Graziano; Erra, Fabrizio; Dini, Fernando; Alimenti, Claudio; Vallesi, Adriana; Pedrini, Bill; Wuthrich, Kurt; Luporini, Pierangelo. "Antarctic and Arctic populations of the ciliate Euplotes nobilii show common pheromone-mediated cell-cell signaling and cross-mating."  Proc. Natl. Acad. Sci. U.S.A. 108, 3181-3186 (2011).

Assembly members:
pheromone En-A1, polymer, 61 residues, 6523.180 Da.

Natural source:   Common Name: ciliates   Taxonomy ID: 184062   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Euplotes nobilii

Experimental source:   Production method: purified from the natural source   Host organism: Euplotes nobilii

Entity Sequences (FASTA):
pheromone En-A1: YNPEDDYTPLTCPHTISVVW YECTENTANCGTACCDSCFE LTGNTMCLLQAGAAGSGCDM E

Data sets:
Data typeCount
13C chemical shifts167
1H chemical shifts371

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1pheromone En-A11

Entities:

Entity 1, pheromone En-A1 61 residues - 6523.180 Da.

1   TYRASNPROGLUASPASPTYRTHRPROLEU
2   THRCYSPROHISTHRILESERVALVALTRP
3   TYRGLUCYSTHRGLUASNTHRALAASNCYS
4   GLYTHRALACYSCYSASPSERCYSPHEGLU
5   LEUTHRGLYASNTHRMETCYSLEULEUGLN
6   ALAGLYALAALAGLYSERGLYCYSASPMET
7   GLU

Samples:

sample_1: En-A1 1.0 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 24.8 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CARA v1.5, Keller and Wuthrich - chemical shift assignment

ATNOS, Herrmann, Guntert and Wuthrich - peak picking

CANDID, Herrmann, Guntert and Wuthrich - collection of distance restraints

CYANA v1.x, Guntert, Mumenthaler and Wuthrich - simulated annealing

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB ACQ66090