BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16477

Title: Solution structure of the transmembrane proximal region of the hepatis C virus E1 glycoprotein.   PubMed: 19891955

Deposition date: 2009-09-04 Original release date: 2009-12-11

Authors: Spadaccini, Roberta; D'Errico, Gerardino; D'Alessio, Viviana; Notomista, Eugenio; Bianchi, Alessia; Merola, Marcello; Picone, Delia

Citation: Spadaccini, Roberta; Notomista, Gerardino; Bianchi, Viviana; Merola, Eugenio; Picone, Alessia. "Structural characterization of the transmembrane proximal region of the hepatitis C virus E1 glycoprotein."  Biochim. Biophys. Acta 1798, 344-353 (2010).

Assembly members:
e1_peptide, polymer, 29 residues, 3328.988 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
e1_peptide: TGHRMAWDMMMNWSPTAALV VAQLLRIPQ

Data sets:
Data typeCount
1H chemical shifts205

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1e11

Entities:

Entity 1, e1 29 residues - 3328.988 Da.

1   THRGLYHISARGMETALATRPASPMETMET
2   METASNTRPSERPROTHRALAALALEUVAL
3   VALALAGLNLEULEUARGILEPROGLN

Samples:

sample_1: e1 1 mM; D2O 20%; hexafluoroisopropanol 80%

sample_2: e1 1 mM; H2O 20%; hexafluoroisopropanol 80%

sample_conditions: pH: 4.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_2isotropicsample_conditions
2D 1H-1H COSYsample_2isotropicsample_conditions
2D 1H-1H NOESYsample_2isotropicsample_conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions
2D 1H-1H COSYsample_1isotropicsample_conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAC41283 BAC41284 BAN91312
EMBL CAA37292 CAD53863 CAD53864 CAD53866 CAD53867
GB AAA45563 AAB02127 AAB02128 AAB66324 AAB67036
REF NP_671491 NP_751920