BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16589

Title: peptide_ptha   PubMed: 20848643

Deposition date: 2009-10-28 Original release date: 2011-05-19

Authors: Neves, Jorge Luiz; Sforca, Mauricio Luis; Murakami, Mario Tyago; Zeri, Ana Carolina de Mattos; Benedetti, Celso Eduardo

Citation: Murakami, Mario Tyago; Sforca, Mauricio Luis; Neves, Jorge Luiz; Paiva, Joice Helena; Domingues, Mariane Noronha; Pereira, Andre Luiz Araujo; Zeri, Ana Carolina de Mattos; Benedetti, Celso Eduardo. "The repeat domain of the type III effector protein PthA shows a TPR-like structure and undergoes conformational changes upon DNA interaction."  Proteins 78, 3386-3395 (2010).

Assembly members:
pthA, polymer, 51 residues, Formula weight is not available

Natural source:   Common Name: Xanthomonas axonopodis pv citri   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Xanthomonas citri

Experimental source:   Production method: obtained from a vendor   Host organism: Xanthomonas axonopodis pv citri

Entity Sequences (FASTA):
pthA: EQVVAIASNIGGKQALETVQ RLLPVLCQAHGLTPEQVVAI ASHDGGKQALE

Data sets:
Data typeCount
1H chemical shifts295

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PthA1

Entities:

Entity 1, PthA 51 residues - Formula weight is not available

1   GLUGLNVALVALALAILEALASERASNILE
2   GLYGLYLYSGLNALALEUGLUTHRVALGLN
3   ARGLEULEUPROVALLEUCYSGLNALAHIS
4   GLYLEUTHRPROGLUGLNVALVALALAILE
5   ALASERHISASPGLYGLYLYSGLNALALEU
6   GLU

Samples:

sample_1: entity 1.0 mM; sodium phosphate 20 mM; SDS 70 mM; DTT 1 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB KHL51201 KHL60368 KHL63085 KOR38772 KOR47463