BMRB Entry 16623
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16623
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Title: The U-box domain of mus musculus E4B PubMed: 20017557
Deposition date: 2009-12-03 Original release date: 2010-01-28
Authors: Nordquist, Kyle; Soss, Sarah; Chazin, Walter
Citation: Nordquist, Kyle; Dimitrova, Yoana; Brzovic, Peter; Ridenour, Whitney; Munro, Kim; Soss, Sarah; Caprioli, Richard; Klevit, Rachel; Chazin, Walter. "Structural and functional characterization of the monomeric U-box domain from E4B." Biochemistry 49, 347-355 (2010).
Assembly members:
E4B, polymer, 85 residues, 9779.069 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
E4B: PGSAEIDYSDAPDEFRDPLM
DTLMTDPVRLPSGTVMDRSI
ILRHLLNSPTDPFNRQMLTE
SMLEPVPELKEQIQAWMREK
QSSDH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 210 |
15N chemical shifts | 76 |
1H chemical shifts | 555 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | E4B | 1 |
Entities:
Entity 1, E4B 85 residues - 9779.069 Da.
1 | PRO | GLY | SER | ALA | GLU | ILE | ASP | TYR | SER | ASP | ||||
2 | ALA | PRO | ASP | GLU | PHE | ARG | ASP | PRO | LEU | MET | ||||
3 | ASP | THR | LEU | MET | THR | ASP | PRO | VAL | ARG | LEU | ||||
4 | PRO | SER | GLY | THR | VAL | MET | ASP | ARG | SER | ILE | ||||
5 | ILE | LEU | ARG | HIS | LEU | LEU | ASN | SER | PRO | THR | ||||
6 | ASP | PRO | PHE | ASN | ARG | GLN | MET | LEU | THR | GLU | ||||
7 | SER | MET | LEU | GLU | PRO | VAL | PRO | GLU | LEU | LYS | ||||
8 | GLU | GLN | ILE | GLN | ALA | TRP | MET | ARG | GLU | LYS | ||||
9 | GLN | SER | SER | ASP | HIS |
Samples:
sample_1: U-box domain of E4B, [U-100% 13C; U-100% 15N], 750 uM; H2O 90%; D2O 10%
sample_2: U-box domain of E4B 1 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2009.015.15.35, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3.113, Goddard - chemical shift assignment, peak picking
CYANA v2..1, Guntert, Mumenthaler and Wuthrich - structure solution
AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
PDB | |
DBJ | BAC26672 BAC56586 BAC65627 |
GB | AAG17287 AAH67402 AAH75620 |
REF | NP_001258127 NP_071305 XP_005353748 XP_005353749 XP_006239449 |
SP | Q9ES00 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts