BMRB Entry 16701
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16701
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal domain of Stt3p PubMed: 20178386
Deposition date: 2010-02-02 Original release date: 2010-03-26
Authors: Huang, Chengdong; Mohanty, Smita
Citation: Huang, Chengdong; Mohanty, Smita. "Challenging the Limit: NMR Assignment of a 31 kDa Helical Membrane Protein" J. Am. Chem. Soc. 132, 3662-3663 (2010).
Assembly members:
the_C-terminal_domain_of_Stt3p, polymer, 273 residues, Formula weight is not available
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
the_C-terminal_domain_of_Stt3p: MGSSHHHHHHSSGLVPRGSH
STWVTRTAYSSPSVVLPSQT
PDGKLALIDDFREAYYWLRM
NSDEDSKVAAWWDYGYQIGG
MADRTTLVDNNTWNNTHIAI
VGKANASPEEKSYEILKEHD
VDYVLVIFGGLIGFGGDDIN
KFLWNIRISEGIWPEEIKER
DFYTAEGEYRVDARASETMR
NSLLYKMSYKDFPQLFNGGQ
ATDRVRQQMITPLDVPPLDY
FDEVFTSENWNVRIYQLKKD
DAQGRTLRDVGELTRSSTKT
RRSIKRPELGLRV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 714 |
| 15N chemical shifts | 236 |
| 1H chemical shifts | 237 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | the C-terminal domain of Stt3p | 1 |
Entities:
Entity 1, the C-terminal domain of Stt3p 273 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | SER | THR | TRP | VAL | THR | ARG | THR | ALA | TYR | SER | ||||
| 4 | SER | PRO | SER | VAL | VAL | LEU | PRO | SER | GLN | THR | ||||
| 5 | PRO | ASP | GLY | LYS | LEU | ALA | LEU | ILE | ASP | ASP | ||||
| 6 | PHE | ARG | GLU | ALA | TYR | TYR | TRP | LEU | ARG | MET | ||||
| 7 | ASN | SER | ASP | GLU | ASP | SER | LYS | VAL | ALA | ALA | ||||
| 8 | TRP | TRP | ASP | TYR | GLY | TYR | GLN | ILE | GLY | GLY | ||||
| 9 | MET | ALA | ASP | ARG | THR | THR | LEU | VAL | ASP | ASN | ||||
| 10 | ASN | THR | TRP | ASN | ASN | THR | HIS | ILE | ALA | ILE | ||||
| 11 | VAL | GLY | LYS | ALA | ASN | ALA | SER | PRO | GLU | GLU | ||||
| 12 | LYS | SER | TYR | GLU | ILE | LEU | LYS | GLU | HIS | ASP | ||||
| 13 | VAL | ASP | TYR | VAL | LEU | VAL | ILE | PHE | GLY | GLY | ||||
| 14 | LEU | ILE | GLY | PHE | GLY | GLY | ASP | ASP | ILE | ASN | ||||
| 15 | LYS | PHE | LEU | TRP | ASN | ILE | ARG | ILE | SER | GLU | ||||
| 16 | GLY | ILE | TRP | PRO | GLU | GLU | ILE | LYS | GLU | ARG | ||||
| 17 | ASP | PHE | TYR | THR | ALA | GLU | GLY | GLU | TYR | ARG | ||||
| 18 | VAL | ASP | ALA | ARG | ALA | SER | GLU | THR | MET | ARG | ||||
| 19 | ASN | SER | LEU | LEU | TYR | LYS | MET | SER | TYR | LYS | ||||
| 20 | ASP | PHE | PRO | GLN | LEU | PHE | ASN | GLY | GLY | GLN | ||||
| 21 | ALA | THR | ASP | ARG | VAL | ARG | GLN | GLN | MET | ILE | ||||
| 22 | THR | PRO | LEU | ASP | VAL | PRO | PRO | LEU | ASP | TYR | ||||
| 23 | PHE | ASP | GLU | VAL | PHE | THR | SER | GLU | ASN | TRP | ||||
| 24 | ASN | VAL | ARG | ILE | TYR | GLN | LEU | LYS | LYS | ASP | ||||
| 25 | ASP | ALA | GLN | GLY | ARG | THR | LEU | ARG | ASP | VAL | ||||
| 26 | GLY | GLU | LEU | THR | ARG | SER | SER | THR | LYS | THR | ||||
| 27 | ARG | ARG | SER | ILE | LYS | ARG | PRO | GLU | LEU | GLY | ||||
| 28 | LEU | ARG | VAL |
Samples:
sample_1: the C-terminal domain of Stt3p, [U-13C; U-15N; U-2H], 600 mM; SDS, [U-2H], 100 mM
sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 328 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Zhengrong and Bax - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Varian INOVA 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAA06079 GAA23364 |
| EMBL | CAA96722 CAY79736 |
| GB | AHY79347 AJP38765 AJR76085 AJR76585 AJR77084 |
| REF | NP_011493 |
| SP | P39007 |
| TPG | DAA08076 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts