BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16742

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Unfolded Bovine Pancreatic Ribonuclease A   PubMed: 20600112

Deposition date: 2010-02-21 Original release date: 2012-08-03

Authors: Azeroual, Simon; Dabrowski, Christian; Denisov, Alexey; Gehring, Kalle

Citation: Kozlov, Guennadi; Azeroual, Simon; Rosenauer, Angelika; Maattanen, Pekka; Denisov, Alexey Yu; Thomas, David; Gehring, Kalle. "Structure of the catalytic a(0)a fragment of the protein disulfide isomerase ERp72."  J. Mol. Biol. 401, 618-625 (2010).

Assembly members:
Ribonuclease A, polymer, 126 residues, Formula weight is not available

Natural source:   Common Name: cattle   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ribonuclease A: MAKETAAAKFERQHMDSSTS AASSSNYCNQMMKSRNLTKD RCKPVNTFVHESLADVQAVC SQKNVACKNGQTNCYQSYST MSITDCRETGSSKYPNCAYK TTQANKHIIVACEGNPYVPV HFDASV

Data sets:
Data typeCount
13C chemical shifts213
15N chemical shifts108
1H chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ribonuclease A1

Entities:

Entity 1, Ribonuclease A 126 residues - Formula weight is not available

1   METALALYSGLUTHRALAALAALALYSPHE
2   GLUARGGLNHISMETASPSERSERTHRSER
3   ALAALASERSERSERASNTYRCYSASNGLN
4   METMETLYSSERARGASNLEUTHRLYSASP
5   ARGCYSLYSPROVALASNTHRPHEVALHIS
6   GLUSERLEUALAASPVALGLNALAVALCYS
7   SERGLNLYSASNVALALACYSLYSASNGLY
8   GLNTHRASNCYSTYRGLNSERTYRSERTHR
9   METSERILETHRASPCYSARGGLUTHRGLY
10   SERSERLYSTYRPROASNCYSALATYRLYS
11   THRTHRGLNALAASNLYSHISILEILEVAL
12   ALACYSGLUGLYASNPROTYRVALPROVAL
13   HISPHEASPALASERVAL

Samples:

sample_1: Bovine Pancreatic Ribonuclease A, [U-100% 13C; U-100% 15N], 0.2 mM; MES, nautral abundance, 25 mM; NaCl, nautral abundance, 70 mM; DTT, nautral abundance, 10 mM; EDTA, nautral abundance, 0.5 mM; H2O, nautral abundance, 90%; D2O, nautral abundance, 10%

sample_conditions_1: ionic strength: 70 mM; pH: 5.5; pressure: 1 atm; temperature: 313.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 1072 16010 16011 16503 17099 17172 19065 2928 385 4031 4032 443
PDB
EMBL CAA30263 CAA33801 CAB37066
GB AAA72269 AAA72757 AAB35594 AAB36134 AAI49530
PIR JC5560 NRBOB
PRF 630436A
REF NP_001014408 XP_005211519 XP_005901936 XP_010837737
SP P61823 P61824
TPE CDG32088
TPG DAA25470

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts