BMRB Entry 16769
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16769
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Title: Solution structure of the par toxin Fst in DPC micelles PubMed: 20677831
Deposition date: 2010-03-08 Original release date: 2010-08-12
Authors: Zangger, Klaus; Gobl, Christoph; Kosol, Simone; Rueckert, Hanna Marie
Citation: Gobl, Christoph; Kosol, Simone; Stockner, Thomas; Ruckert, Hanna; Zangger, Klaus. "Solution structure and membrane binding of the toxin fst of the par addiction module." Biochemistry 49, 6567-6575 (2010).
Assembly members:
Fst, polymer, 33 residues, 3767.501 Da.
Natural source: Common Name: E. faecalis Taxonomy ID: 1351 Superkingdom: Bacteria Kingdom: not available Genus/species: Enterococcus faecalis
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Fst: MKDLMSLVIAPIFVGLVLEM
ISRVLDEEDDSRK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 193 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fst | 1 |
Entities:
Entity 1, Fst 33 residues - 3767.501 Da.
| 1 | MET | LYS | ASP | LEU | MET | SER | LEU | VAL | ILE | ALA | ||||
| 2 | PRO | ILE | PHE | VAL | GLY | LEU | VAL | LEU | GLU | MET | ||||
| 3 | ILE | SER | ARG | VAL | LEU | ASP | GLU | GLU | ASP | ASP | ||||
| 4 | SER | ARG | LYS |
Samples:
sample_1: Fst_toxin .5 mM; potassium phosphate 50 mM; DPC, [U-99% 2H], 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.15 M; pH: 5.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Varian Unity 600 MHz
- Bruker Avance 500 MHz
Related Database Links:
| PDB | |
| GB | AAF03910 EEI55805 EEN72987 EEU27112 EOE77546 |
| REF | WP_002387930 YP_009074128 |