BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16783

Title: Smr in bicelles   PubMed: 20407887

Deposition date: 2010-03-22 Original release date: 2010-05-03

Authors: Poget, Sebastien; Richard, Harris; Sean, Cahill

Citation: Poget, Sebastien; Harris, Richard; Cahill, Sean; Girvin, Mark. "1H, 13C, 15N backbone NMR assignments of the Staphylococcus aureus small multidrug-resistance pump (Smr) in a functionally active conformation."  Biomol. NMR Assignments 4, 139-142 (2010).

Assembly members:
Smr, polymer, 107 residues, Formula weight is not available

Natural source:   Common Name: firmicutes   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Smr: MPYIYLIIAISTEVIGSAFL KSSEGFSKFIPSLGTIISFG ICFYFLSKTMQHLPLNITYA TWAGLGLVLTTVVSIIIFKE QINLITIVSIVLIIVGVVSL NIFGTSH

Data sets:
Data typeCount
13C chemical shifts244
15N chemical shifts86
1H chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer 11
2monomer 21

Entities:

Entity 1, monomer 1 107 residues - Formula weight is not available

1   METPROTYRILETYRLEUILEILEALAILE
2   SERTHRGLUVALILEGLYSERALAPHELEU
3   LYSSERSERGLUGLYPHESERLYSPHEILE
4   PROSERLEUGLYTHRILEILESERPHEGLY
5   ILECYSPHETYRPHELEUSERLYSTHRMET
6   GLNHISLEUPROLEUASNILETHRTYRALA
7   THRTRPALAGLYLEUGLYLEUVALLEUTHR
8   THRVALVALSERILEILEILEPHELYSGLU
9   GLNILEASNLEUILETHRILEVALSERILE
10   VALLEUILEILEVALGLYVALVALSERLEU
11   ASNILEPHEGLYTHRSERHIS

Samples:

sample_1: Smr, [U-100% 13C; U-100% 15N; U-100% 2H], 1 mM; DHPC 200 mM; DMPC 84 mM; potassium phosphate 20 mM; TSP 1 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 315.5 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
2D CACOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

DBJ BAD01019 BAE92298 BAP94573
EMBL CAA33598 CAA44471 CAA44472 CAA86016 CAC14916
GB AAA26666 AAA26668 AAA60379 AAB02114 AAB07747
PRF 1907159A
REF NP_647561 NP_863640 NP_878005 NP_940784 WP_001146389
SP P14319 Q55339

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts