BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16854

Title: an eye lens fragment from human alpha-crystallin

Deposition date: 2010-04-11 Original release date: 2012-08-02

Authors: Chary, Kandala; Srivastava, Atul

Citation: Chary, Kandala; Srivastava, Atul. "NMR resonance assignment of an eye lens fragment from huma aloha crystallin"  Not known ., .-..

Assembly members:
fragment, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
fragment: SDRDKFVIFLDVKHF

Data sets:
Data typeCount
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1eye lens fragment1

Entities:

Entity 1, eye lens fragment 15 residues - Formula weight is not available

1   SERASPARGASPLYSPHEVALILEPHELEU
2   ASPVALLYSHISPHE

Samples:

sample_1: fragment 700 uM; H2O 90%; D2O 10%

sample_2: fragment 700 uM; D2O 100%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - processing

TOPSPIN, Bruker Biospin - collection

CARA, http://www.nmr.ch/doku.php - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA95535 BAE25387
EMBL CAA24530 CAA26696 CAA32891 CAA64668 CAC35358
GB AAA30471 AAA37469 AAA37470 AAA37471 AAA66165
PIR CYGCAA
PRF 1504307A 2021294A 751000A 751000B 751000C
REF NP_000385 NP_001012476 NP_001074367 NP_001075875 NP_001157483
SP P02470 P02472 P02474 P02475 P02476
TPG DAA32986