BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16960

Title: Solution structure of GxTX-1E   PubMed: 20509680

Deposition date: 2010-05-27 Original release date: 2010-07-26

Authors: Lee, Seungkyu; Jung, Hyun Ho; Lee, Ju Yeon; Lee, Chul Won; Kim, Jae Il

Citation: Lee, Seungkyu; Milescu, Mirela; Jung, Hyun Ho; Lee, Ju Yeon; Bae, Chan Hyung; Lee, Chul Won; Kim, Ha Hyung; Swartz, Kenton; Kim, Jae Il. "Solution structure of GxTX-1E, a high-affinity tarantula toxin interacting with voltage sensors in Kv2.1 potassium channels ."  Biochemistry 49, 5134-5142 (2010).

Assembly members:
GxTX-1E, polymer, 36 residues, 3948.66 Da.

Natural source:   Common Name: spiders   Taxonomy ID: 278060   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Plesiophrictus guangxiensis

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
GxTX-1E: EGECGGFWWKCGSGKPACCP KYVCSPKWGLCNFPMP

Data sets:
Data typeCount
1H chemical shifts206

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GxTX-1E1

Entities:

Entity 1, GxTX-1E 36 residues - 3948.66 Da.

1   GLUGLYGLUCYSGLYGLYPHETRPTRPLYS
2   CYSGLYSERGLYLYSPROALACYSCYSPRO
3   LYSTYRVALCYSSERPROLYSTRPGLYLEU
4   CYSASNPHEPROMETPRO

Samples:

sample_1: GxTX-1E 10 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.500; temperature: 310.000 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1solutionsample_conditions_1
TOCSYsample_1solutionsample_conditions_1
NOESYsample_1solutionsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS1.2 vany, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

SPARKY vany, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 0 MHz

Related Database Links:

PDB
GB ABY71685 ABY71686
SP B1P1D5 B1P1D6 P84835 P84836