BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17034

Title: 1H and 15N Chemical Shift Assignments for the Syrian hamster prion protein shPrP(90-232)   PubMed: 21189021

Deposition date: 2010-06-30 Original release date: 2011-01-19

Authors: Bjorndahl, Trent; Zhou, Guo-Ping; Lui, Xue-Hui; Perez-Pineiro, Rolando; Semenchenko, Valentyna; Bujold, Adina; Sobsey, Constance; Wishart, David

Citation: Bjorndahl, Trent; Zhou, Guo-Ping; Liu, Xuehui; Perez-Pineiro, Rolando; Semenchenko, Valentyna; Saleem, Fozia; Acharya, Sandipta; Bujold, Adina; Sobsey, Constance; Wishart, David. "Detailed Biophysical Characterization of the Acid-Induced PrP(c) to PrP() Conversion Process."  Biochemistry 50, 1162-1173 (2011).

Assembly members:
shPrP, polymer, 162 residues, 18710.7 Da.

Natural source:   Common Name: Golden hamster   Taxonomy ID: 10036   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesocricetus auratus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
shPrP: MGSSHHHHHHSSGLVPRGSG QGGGTHNQWNKPSKPKTNMK HMAGAAAAGAVVGGLGGYML GSAMSRPMMHFGNDWEDRYY RENMNRYPNQVYYRPVDQYN NQNNFVHDCVNITIKQHTVT TTTKGENFTETDIKIMERVV EQMCTTQYQKESQAYYDGRR SS

Data typeCount
15N chemical shifts544
1H chemical shifts1791

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1shPrP(90-232)1

Entities:

Entity 1, shPrP(90-232) 162 residues - 18710.7 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERGLY
3   GLNGLYGLYGLYTHRHISASNGLNTRPASN
4   LYSPROSERLYSPROLYSTHRASNMETLYS
5   HISMETALAGLYALAALAALAALAGLYALA
6   VALVALGLYGLYLEUGLYGLYTYRMETLEU
7   GLYSERALAMETSERARGPROMETMETHIS
8   PHEGLYASNASPTRPGLUASPARGTYRTYR
9   ARGGLUASNMETASNARGTYRPROASNGLN
10   VALTYRTYRARGPROVALASPGLNTYRASN
11   ASNGLNASNASNPHEVALHISASPCYSVAL
12   ASNILETHRILELYSGLNHISTHRVALTHR
13   THRTHRTHRLYSGLYGLUASNPHETHRGLU
14   THRASPILELYSILEMETGLUARGVALVAL
15   GLUGLNMETCYSTHRTHRGLNTYRGLNLYS
16   GLUSERGLNALATYRTYRASPGLYARGARG
17   SERSER

Samples:

sample_1: shPrP, [U-100% 15N], 0.5 – 1.0 mM; sodium acetate 20 mM; H2O 90%; D2O 10%

sample_2: shPrP, [U-100% 15N], 0.5 – 1.0 mM; sodium acetate 20 mM; H2O 90%; D2O 10%

sample_3: shPrP, [U-100% 15N], 0.5 – 1.0 mM; sodium acetate 20 mM; H2O 90%; D2O 10%

sample_4: shPrP, [U-100% 15N], 0.5 – 1.0 mM; Formic Acetate 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 5.2; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 0.02 M; pH: 3.5; pressure: 1 atm; temperature: 303 K

sample_conditions_3: ionic strength: 0.02 M; pH: 3.2; pressure: 1 atm; temperature: 303 K

sample_conditions_4: ionic strength: 0.02 M; pH: 3.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D HNHAsample_2isotropicsample_conditions_2
3D 1H-15N TOCSYsample_2isotropicsample_conditions_2
3D 1H-15N NOESYsample_2isotropicsample_conditions_2
2D 1H-15N HSQCsample_3isotropicsample_conditions_3
3D HNHAsample_3isotropicsample_conditions_3
3D 1H-15N TOCSYsample_3isotropicsample_conditions_3
3D 1H-15N NOESYsample_3isotropicsample_conditions_3
2D 1H-15N HSQCsample_4isotropicsample_conditions_4
3D HNHAsample_4isotropicsample_conditions_4
3D 1H-15N TOCSYsample_4isotropicsample_conditions_4
3D 1H-15N NOESYsample_4isotropicsample_conditions_4

Software:

NMRView vNMRViewJ v. 8.0.a21, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ v2.1b, Varian - collection

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 17834 4307
PDB
GB AAA37090 AAA37091 AAA37092 AAA37093 AAB25731
REF XP_005068717 XP_012967855
SP P04273

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts