BMRB Entry 17036
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17036
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: SOLUTION STRUCTURE OF ZINC FINGER DOMAIN OF E3 UBIQUITIN-PROTEIN LIGASE PROTEIN Praja-1 FROM HOMO SAPIENS, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET HR4710B
Deposition date: 2010-06-30 Original release date: 2010-08-24
Authors: Liu, Gaohua; Tong, Sai; Hamilton, Keith; Ciccosanti, Colleen; Shastry, Ritu; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano
Citation: Liu, Gaohua; Tong, Sai; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano. "SOLUTION STRUCTURE OF ZINC FINGER DOMAIN OF E3 UBIQUITIN-PROTEIN LIGASE PROTEIN Praja-1 FROM HOMO SAPIENS, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET HR4710B" Not known ., .-..
Assembly members:
pja1_zinc_finger_domain, polymer, 91 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
pja1_zinc_finger_domain: MGHHHHHHSHMVANPPASKE
SIDALPEILVTEDHGAVGQE
MCCPICCSEYVKGDVATELP
CHHYFHKPCVSIWLQKSGTC
PVCRCMFPPPL
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 277 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 554 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pja1 zinc finger domain | 1 |
Entities:
Entity 1, pja1 zinc finger domain 91 residues - Formula weight is not available
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | SER | HIS | ||||
| 2 | MET | VAL | ALA | ASN | PRO | PRO | ALA | SER | LYS | GLU | ||||
| 3 | SER | ILE | ASP | ALA | LEU | PRO | GLU | ILE | LEU | VAL | ||||
| 4 | THR | GLU | ASP | HIS | GLY | ALA | VAL | GLY | GLN | GLU | ||||
| 5 | MET | CYS | CYS | PRO | ILE | CYS | CYS | SER | GLU | TYR | ||||
| 6 | VAL | LYS | GLY | ASP | VAL | ALA | THR | GLU | LEU | PRO | ||||
| 7 | CYS | HIS | HIS | TYR | PHE | HIS | LYS | PRO | CYS | VAL | ||||
| 8 | SER | ILE | TRP | LEU | GLN | LYS | SER | GLY | THR | CYS | ||||
| 9 | PRO | VAL | CYS | ARG | CYS | MET | PHE | PRO | PRO | PRO | ||||
| 10 | LEU |
Samples:
sample_NC: pja1 zinc finger domain, [U-100% 13C; U-100% 15N], 0.94 mM; H2O 90%; D2O 10%
sample_nc5: pja1 zinc finger domain, [U-10% 13C; U-100% 15N], 0.82 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_NC | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_nc5 | isotropic | sample_conditions_1 |
| 3D 1H-13C-15N SIMUTANEOUS NOESY | sample_NC | isotropic | sample_conditions_1 |
| 3D 1H-13C aromatic NOESY | sample_NC | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_NC | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_NC | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_NC | isotropic | sample_conditions_1 |
| 3D HNCO | sample_NC | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_NC | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_NC | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinement, structure solution
CYANA, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinement, structure solution
AutoStruct, Huang, Tejero, Powers and Montelione - data analysis, geometry optimization, refinement
TALOS+, Shen, Delaglio and Bax - data analysis
XEASY, Bartels et al. - data analysis, peak picking, refinement
AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
VNMRJ, Varian - collection
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB13928 BAB23982 BAE22512 BAE73073 BAE88506 |
| EMBL | CAH93231 |
| GB | AAC00205 AAH08148 AAH25975 AAH37616 AAH48323 |
| REF | NP_001027568 NP_001076579 NP_001094476 NP_001128898 NP_001277484 |
| SP | O55176 Q8NG27 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts