BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17050

Title: Tudor domain from Drosophila Polycomblike   PubMed: 20669242

Deposition date: 2010-07-08 Original release date: 2010-08-12

Authors: Friberg, A.; Oddone, A.; Klymenko, T.; Mueller, J.; Sattler, M.

Citation: Friberg, Anders; Oddone, Anna; Klymenko, Tetyana; Muller, Jurg; Sattler, Michael. "Structure of an atypical Tudor domain in the Drosophila Polycomblike protein."  Protein Sci. 19, 1906-1916 (2010).

Assembly members:
POLYCOMB_PROTEIN_PCL, polymer, 69 residues, 7578.4194 Da.

Natural source:   Common Name: FRUIT FLY   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
POLYCOMB_PROTEIN_PCL: GAMAPPVAAPSPAVTYALQE DVFIKCNDGRFYLGTIIDQT SDQYLIRFDDQSEQWCEPDK LRKLGGGSS

Data sets:
Data typeCount
13C chemical shifts220
15N chemical shifts66
1H chemical shifts442

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1POLYCOMB PROTEIN PCL1

Entities:

Entity 1, POLYCOMB PROTEIN PCL 69 residues - 7578.4194 Da.

Three first residues is a cloning artifact.

1   GLYALAMETALAPROPROVALALAALAPRO
2   SERPROALAVALTHRTYRALALEUGLNGLU
3   ASPVALPHEILELYSCYSASNASPGLYARG
4   PHETYRLEUGLYTHRILEILEASPGLNTHR
5   SERASPGLNTYRLEUILEARGPHEASPASP
6   GLNSERGLUGLNTRPCYSGLUPROASPLYS
7   LEUARGLYSLEUGLYGLYGLYSERSER

Samples:

sample_1: POLYCOMB PROTEIN PCL, [U-13C; U-15N], 0.25 – 1.0 mM; salt 25 mM; buffer 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 25.000 mM; pH: 6.300; pressure: 1.000 atm; temperature: 298.500 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CBCA(CO)NHsample_1solutionsample_conditions_1
(H)CC(CO)NH-TOCSYsample_1solutionsample_conditions_1
H(CC)(CO)NH-TOCSYsample_1solutionsample_conditions_1
H(C)CH-TOCSYsample_1solutionsample_conditions_1
(HB)CB(CGCD)HDsample_1solutionsample_conditions_1
(HB)CB(CGCDCE)HEsample_1solutionsample_conditions_1
1H-15N HSQC-NOESYsample_1solutionsample_conditions_1
1H-13C HMQC-NOESYsample_1solutionsample_conditions_1

Software:

CNS vany, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - refinement

NMRView vany, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DMX 0 MHz

Related Database Links:

UNP PCL_DROME
PDB
GB AAA64457 AAF57748 AAL68389 ACL83738 ACL88860
REF NP_001261067 NP_476672 XP_001974621 XP_002034393 XP_002082031
SP Q24459

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts