BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17358

Title: Solution structure of the third Immunoglobulin-like domain of nectin-1

Deposition date: 2010-12-13 Original release date: 2012-02-15

Authors: Clausen, Ole; Poulsen, Flemming

Citation: Clausen, Ole; Poulsen, Flemming. "Solution structure of the third Immunoglobulin-like domain of nectin-1"  Not known ., .-..

Assembly members:
entity, polymer, 95 residues, 10599.844 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
entity: LNVQYEPEVTIEGFDGNWYL QRTDVKLTCKADANPPATEY HWTTLNGSLPKGVEAQNRTL FFRGPITYSLAGTYICEATN PIGTRSGQVEVNITH

Data sets:
Data typeCount
13C chemical shifts170
15N chemical shifts84
1H chemical shifts373

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nectin-11

Entities:

Entity 1, nectin-1 95 residues - 10599.844 Da.

1   LEUASNVALGLNTYRGLUPROGLUVALTHR
2   ILEGLUGLYPHEASPGLYASNTRPTYRLEU
3   GLNARGTHRASPVALLYSLEUTHRCYSLYS
4   ALAASPALAASNPROPROALATHRGLUTYR
5   HISTRPTHRTHRLEUASNGLYSERLEUPRO
6   LYSGLYVALGLUALAGLNASNARGTHRLEU
7   PHEPHEARGGLYPROILETHRTYRSERLEU
8   ALAGLYTHRTYRILECYSGLUALATHRASN
9   PROILEGLYTHRARGSERGLYGLNVALGLU
10   VALASNILETHRHIS

Samples:

sample_1: nectin-1, [U-13C; U-15N], 1.0 mM; sodium phosphate 10 mM; sodium chloride 50 mM; sodium azide 0.02 % v/v

sample_conditions_1: ionic strength: 0.06 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PRONTO, Kjur, Andersen and Poulsen - chemical shift assignment, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

ARIA, Linge, O, . - refinement

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
GB AAF60333 AAF76195 AAG22808 AAG30283 AAH60694
REF NP_001093946 NP_067399 XP_003513871 XP_004667415 XP_005069470
SP Q9JKF6

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts