BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17376

Title: Backbone Assignment of Human Proliferating Cell Nuclear Antigen Complexed with a 12 Amino Acids Peptide from the C-terminal Region of p21 (WAF1/CIP1)   PubMed: 23139781

Deposition date: 2010-12-22 Original release date: 2012-11-26

Authors: Lopez-Alonso, Jorge; Sanchez, Ricardo; Blanco, Francisco; Campos-Olivas, Ramon

Citation: De Biasio, Alfredo; Campos-Olivas, Ramon; Sanchez, Ricardo; Lopez-Alonso, Jorge; Pantoja-Uceda, David; Merino, Nekane; Villate, Maider; Martin-Garcia, Jose; Castillo, Francisco; Luque, Irene; Blanco, Francisco. "Proliferating Cell Nuclear Antigen (PCNA) Interactions in Solution Studied by NMR"  PLoS One 7, e48390-e48390 (2012).

Assembly members:
hPCNA, polymer, 264 residues, Formula weight is not available
p2112, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hPCNA: GPHMFEARLVQGSILKKVLE ALKDLINEACWDISSSGVNL QSMDSSHVSLVQLTLRSEGF DTYRCDRNLAMGVNLTSMSK ILKCAGNEDIITLRAEDNAD TLALVFEAPNQEKVSDYEMK LMDLDVEQLGIPEQEYSCVV KMPSGEFARICRDLSHIGDA VVISCAKDGVKFSASGELGN GNIKLSQTSNVDKEEEAVTI EMNEPVQLTFALRYLNFFTK ATPLSSTVTLSMSADVPLVV EYKIADMGHLKYYLAPKIED EEGS
p2112: KRRQTSMTDFYH

Data sets:
Data typeCount
13C chemical shifts676
15N chemical shifts258
1H chemical shifts276

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hPCNA monomer 11
2hPCNA monomer 21
3hPCNA monomer 31
4P2112 molecule 12
5P2112 molecule 22
6P2112 molecule 32

Entities:

Entity 1, hPCNA monomer 1 264 residues - Formula weight is not available

Residues -3 to -1 are a non-native tag. The native sequence starts at residue number 4: MF...

1   GLYPROHISMETPHEGLUALAARGLEUVAL
2   GLNGLYSERILELEULYSLYSVALLEUGLU
3   ALALEULYSASPLEUILEASNGLUALACYS
4   TRPASPILESERSERSERGLYVALASNLEU
5   GLNSERMETASPSERSERHISVALSERLEU
6   VALGLNLEUTHRLEUARGSERGLUGLYPHE
7   ASPTHRTYRARGCYSASPARGASNLEUALA
8   METGLYVALASNLEUTHRSERMETSERLYS
9   ILELEULYSCYSALAGLYASNGLUASPILE
10   ILETHRLEUARGALAGLUASPASNALAASP
11   THRLEUALALEUVALPHEGLUALAPROASN
12   GLNGLULYSVALSERASPTYRGLUMETLYS
13   LEUMETASPLEUASPVALGLUGLNLEUGLY
14   ILEPROGLUGLNGLUTYRSERCYSVALVAL
15   LYSMETPROSERGLYGLUPHEALAARGILE
16   CYSARGASPLEUSERHISILEGLYASPALA
17   VALVALILESERCYSALALYSASPGLYVAL
18   LYSPHESERALASERGLYGLULEUGLYASN
19   GLYASNILELYSLEUSERGLNTHRSERASN
20   VALASPLYSGLUGLUGLUALAVALTHRILE
21   GLUMETASNGLUPROVALGLNLEUTHRPHE
22   ALALEUARGTYRLEUASNPHEPHETHRLYS
23   ALATHRPROLEUSERSERTHRVALTHRLEU
24   SERMETSERALAASPVALPROLEUVALVAL
25   GLUTYRLYSILEALAASPMETGLYHISLEU
26   LYSTYRTYRLEUALAPROLYSILEGLUASP
27   GLUGLUGLYSER

Entity 2, P2112 molecule 1 12 residues - Formula weight is not available

1   LYSARGARGGLNTHRSERMETTHRASPPHE
2   TYRHIS

Samples:

sample_1: hPCNA, [U-13C; U-15N], 1.0 mM; p2112 1.5 mM; sodium chloride 137 mM; sodium phosphate 2.7 mM; potassium chloride 10 mM; potassium phosphate 2 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.327 M; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D trHNCACBsample_1isotropicsample_conditions_1
3D trHNCOsample_1isotropicsample_conditions_1
4D 1H-15N,1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15501 17375
PDB
DBJ BAB28355 BAB28557 BAC40240 BAE30230 BAE30376
EMBL CAA40938 CAA68261 CAG38740 CAG46598
GB AAA35736 AAA60040 AAG10077 AAG37435 AAH00491
REF NP_001029666 NP_001233697 NP_001253019 NP_001274649 NP_001278854
SP P04961 P12004 P17918 P57761 P61258
TPG DAA23435

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts