BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17385

Title: TEX13A RanBP2 zinc finger backbone chemical shifts   PubMed: 21256132

Deposition date: 2010-12-23 Original release date: 2011-01-28

Authors: Mansfield, Robyn; Mackay, Joel; Nguyen, Cuong

Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA."  J. Mol. Biol. 407, 273-283 (2011).

Assembly members:
TEX13A_ZnF, polymer, 42 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TEX13A_ZnF: GSRPPVYRRPGDWDCPWCNA VNFSRRDTCFDCGKGIWLQK PH

Data sets:
Data typeCount
13C chemical shifts71
15N chemical shifts33
1H chemical shifts33

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein component1
2zinc ion2

Entities:

Entity 1, protein component 42 residues - Formula weight is not available

N-terminal GS is non-native, resulting from cleavage of a GST tag.

1   GLYSERARGPROPROVALTYRARGARGPRO
2   GLYASPTRPASPCYSPROTRPCYSASNALA
3   VALASNPHESERARGARGASPTHRCYSPHE
4   ASPCYSGLYLYSGLYILETRPLEUGLNLYS
5   PROHIS

Entity 2, zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: TEX13A ZnF, [U-100% 13C; U-100% 15N], 1 mM; DTT 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; DSS 20 uM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.125 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

GB AAH42547 AAI08735 AAK31976 ABZ92542 ADZ15641
REF NP_001278206 NP_112564 XP_001092375 XP_002831997 XP_003317653
SP Q9BXU3

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts