BMRB Entry 17386
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17386
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Title: RBM10 RanBP2 zinc finger backbone chemical shifts PubMed: 21256132
Deposition date: 2010-12-23 Original release date: 2011-01-28
Authors: Mansfield, Robyn; Mackay, Joel; Nguyen, Cuong
Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA." J. Mol. Biol. 407, 273-283 (2011).
Assembly members:
RBM10_ZnF, polymer, 41 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RBM10_ZnF: GSSDPKPKINEDWLCNKCGV
QNFKRREKCFKCGVPKSEAE
Q
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 76 |
15N chemical shifts | 33 |
1H chemical shifts | 33 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | protein component | 1 |
2 | zinc ion | 2 |
Entities:
Entity 1, protein component 41 residues - Formula weight is not available
N-terminal GS is non-native, resulting from cleavage of a GST tag.
1 | GLY | SER | SER | ASP | PRO | LYS | PRO | LYS | ILE | ASN | ||||
2 | GLU | ASP | TRP | LEU | CYS | ASN | LYS | CYS | GLY | VAL | ||||
3 | GLN | ASN | PHE | LYS | ARG | ARG | GLU | LYS | CYS | PHE | ||||
4 | LYS | CYS | GLY | VAL | PRO | LYS | SER | GLU | ALA | GLU | ||||
5 | GLN |
Entity 2, zinc ion - Zn - 65.409 Da.
1 | ZN |
Samples:
sample_1: RBM10 ZnF, [U-100% 13C; U-100% 15N], 1 mM; DTT 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; DSS 20 uM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0.125 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
BMRB | 25433 |
PDB | |
DBJ | BAA09471 BAA12144 BAC31087 BAC40753 BAC65490 |
EMBL | CAD97933 CAL37951 |
GB | AAH00681 AAH03089 AAH04181 AAH04674 AAH08733 |
REF | NP_001161247 NP_001161248 NP_001191395 NP_001191396 NP_001191397 |
SP | P70501 P98175 Q99KG3 |
TPG | DAA12902 DAA12903 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts