BMRB Entry 17559
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17559
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Title: Assignment of the stem loop 2 of RsmZ PubMed: 22252483
Deposition date: 2011-03-31 Original release date: 2012-01-24
Authors: Schubert, Mario; Aeschbacher, Thomas; Duss, Olivier; Allain, Frederic
Citation: Aeschbacher, Thomas; Schubert, Mario; Allain, Frederic H-T. "A procedure to validate and correct the (13)C chemical shift calibration of RNA datasets." J. Biomol. NMR 52, 179-190 (2012).
Assembly members:
FZL2, polymer, 22 residues, Formula weight is not available
Natural source: Common Name: g-proteobacteria Taxonomy ID: 294 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas fluorescens
Experimental source: Production method: cell free synthesis Host organism: not applicable
Entity Sequences (FASTA):
FZL2: GGGCCAUCAAGGACGAUGGU
CC
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 59 |
1H chemical shifts | 59 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | FZL2 (monomer) | 1 |
Entities:
Entity 1, FZL2 (monomer) 22 residues - Formula weight is not available
1 | G | G | G | C | C | A | U | C | A | A | ||||
2 | G | G | A | C | G | A | U | G | G | U | ||||
3 | C | C |
Samples:
sample_1: FZL2 2 mM; D2O 100%
sample_2: FZL2 2 mM; D2O 5%; H2O 95%
sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, refinement
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 900 MHz