BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17617

Title: IIIS4

Deposition date: 2011-05-02 Original release date: 2011-06-03

Authors: Darbon, Herv; De waar, Michel

Citation: Douzi, Badreddine; Darbon, Herve; De waar, Michel. "structure of S4-3 voltage sensor peptide"  J. Biol. Chem. ., .-..

Assembly members:
Peptide, polymer, 28 residues, 3351.292 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Peptide: KDINTIKSLRVLRVLRPLKT IKRLPKLK

Data sets:
Data typeCount
1H chemical shifts236

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S4-3 voltage sensor peptide1

Entities:

Entity 1, S4-3 voltage sensor peptide 28 residues - 3351.292 Da.

1   LYSASPILEASNTHRILELYSSERLEUARG
2   VALLEUARGVALLEUARGPROLEULYSTHR
3   ILELYSARGLEUPROLYSLEULYS

Samples:

sample_1: Peptide 1 mM; D2O 10 mM; H2O 90 mM; Trifluoroethanol 30%

sample_conditions_1: pH: 4; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift calculation

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAA94765 BAA94766 BAB85611 BAB85612 BAD92704
EMBL CAA40714 CAA40715 CAA40871 CAA40872 CAA68172
GB AAA40806 AAA42014 AAA51897 AAA51898 AAB49674
PRF 1709354A 1709354B
REF NP_000059 NP_000709 NP_001035993 NP_001068597 NP_001095163
SP O00555 O55017 P27884 P54282 P56699
TPG DAA28081