BMRB Entry 17815
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17815
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Title: Backbone 1H, 13C and 15N resonance assignments for IMP1 KH34 PubMed: 22215810
Deposition date: 2011-07-29 Original release date: 2012-04-23
Authors: Harris, Richard; Patel, Vivek; Chao, Jeffery; Girvin, Mark; Singer, Robert
Citation: Patel, Vivek; Mitra, Somdeb; Harris, Richard; Buxbaum, Adina; Lionnet, Timothee; Brenowitz, Michael; Girvin, Mark; Levy, Matthew; Almo, Steven; Singer, Robert; Chao, Jeffery. "Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control" Genes Dev. 26, 43-53 (2012).
Assembly members:
IMP1_KH34, polymer, 172 residues, 19196 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
IMP1_KH34: MEQEMVQVFIPAQAVGAIIG
KKGQHIKQLSRFASASIKIA
PPETPDSKVRMVIITGPPEA
QFKAQGRIYGKLKEENFFGP
KEEVKLETHIRVPASAAGRV
IGKGGKTVNELQNLTAAEVV
VPRDQTPDENDQVIVKIIGH
FYASQMAQRKIRDILAQVKQ
QHQKLEHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 428 |
15N chemical shifts | 127 |
1H chemical shifts | 127 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | IMP1 KH34 | 1 |
Entities:
Entity 1, IMP1 KH34 172 residues - 19196 Da.
Residues M(403) and L(567)-H(574) are cloning artifacts and C-terminal His-tag
1 | MET | GLU | GLN | GLU | MET | VAL | GLN | VAL | PHE | ILE | ||||
2 | PRO | ALA | GLN | ALA | VAL | GLY | ALA | ILE | ILE | GLY | ||||
3 | LYS | LYS | GLY | GLN | HIS | ILE | LYS | GLN | LEU | SER | ||||
4 | ARG | PHE | ALA | SER | ALA | SER | ILE | LYS | ILE | ALA | ||||
5 | PRO | PRO | GLU | THR | PRO | ASP | SER | LYS | VAL | ARG | ||||
6 | MET | VAL | ILE | ILE | THR | GLY | PRO | PRO | GLU | ALA | ||||
7 | GLN | PHE | LYS | ALA | GLN | GLY | ARG | ILE | TYR | GLY | ||||
8 | LYS | LEU | LYS | GLU | GLU | ASN | PHE | PHE | GLY | PRO | ||||
9 | LYS | GLU | GLU | VAL | LYS | LEU | GLU | THR | HIS | ILE | ||||
10 | ARG | VAL | PRO | ALA | SER | ALA | ALA | GLY | ARG | VAL | ||||
11 | ILE | GLY | LYS | GLY | GLY | LYS | THR | VAL | ASN | GLU | ||||
12 | LEU | GLN | ASN | LEU | THR | ALA | ALA | GLU | VAL | VAL | ||||
13 | VAL | PRO | ARG | ASP | GLN | THR | PRO | ASP | GLU | ASN | ||||
14 | ASP | GLN | VAL | ILE | VAL | LYS | ILE | ILE | GLY | HIS | ||||
15 | PHE | TYR | ALA | SER | GLN | MET | ALA | GLN | ARG | LYS | ||||
16 | ILE | ARG | ASP | ILE | LEU | ALA | GLN | VAL | LYS | GLN | ||||
17 | GLN | HIS | GLN | LYS | LEU | GLU | HIS | HIS | HIS | HIS | ||||
18 | HIS | HIS |
Samples:
sample_1: IMP1 KH34, [U-13C; U-15N; U-2H], 250 uM; TRIS 25 mM; sodium chloride 300 mM; DSS 1 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 300 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CB | sample_1 | isotropic | sample_conditions_1 |
3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ v2.2D, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CCPN_Analysis v2.1.2, CCPN - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
PDB | |
DBJ | BAG52029 |
GB | ABB46294 EHH57936 KFP20603 KFZ57551 |
REF | NP_001153895 XP_002800442 XP_003278799 XP_003414705 XP_003435309 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts