BMRB Entry 17917
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17917
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Title: Backbone chemical shift assignments for the C-terminal domain of yeast Tfg1. PubMed: 22095626
Deposition date: 2011-09-06 Original release date: 2011-12-14
Authors: Kilpatrick, Adina; Koharudin, Leonardus; Gronenborn, Angela
Citation: Kilpatrick, Adina; Koharudin, Leonardus; Calero, Guillermo; Gronenborn, Angela. "Structural and binding studies of the C-terminal domains of yeast TFIIF subunits Tfg1 and Tfg2." Proteins ., .-. (2011).
Assembly members:
Tfg1_C-terminal_domain, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Tfg1_C-terminal_domain: MASTITEKDIIEAIGDGKVN
IKEFGKFIRRKYPGAENKKL
MFAIVKKLCRKVGNDHMELK
KE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 179 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 124 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tfg1 | 1 |
Entities:
Entity 1, Tfg1 62 residues - Formula weight is not available
| 1 | MET | ALA | SER | THR | ILE | THR | GLU | LYS | ASP | ILE | ||||
| 2 | ILE | GLU | ALA | ILE | GLY | ASP | GLY | LYS | VAL | ASN | ||||
| 3 | ILE | LYS | GLU | PHE | GLY | LYS | PHE | ILE | ARG | ARG | ||||
| 4 | LYS | TYR | PRO | GLY | ALA | GLU | ASN | LYS | LYS | LEU | ||||
| 5 | MET | PHE | ALA | ILE | VAL | LYS | LYS | LEU | CYS | ARG | ||||
| 6 | LYS | VAL | GLY | ASN | ASP | HIS | MET | GLU | LEU | LYS | ||||
| 7 | LYS | GLU |
Samples:
sample_1: Tfg1 C-terminal domain, [U-98% 13C; U-98% 15N], 1 – 1.5 mM; sodium phosphate 20 mM; sodium chloride 100 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - data analysis, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
Related Database Links:
| DBJ | GAA23558 |
| EMBL | CAA67530 CAA97212 CAY79945 |
| GB | AAA61640 AAA68478 AHY79527 AJP38957 AJR76277 |
| REF | NP_011702 |
| SP | P41895 |
| TPG | DAA08280 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts