BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17987

Title: New tricks of an old fold: structural versatility of scorpion toxins with common cysteine spacing   PubMed: 22238341

Deposition date: 2011-10-09 Original release date: 2012-01-18

Authors: del Rio-Portilla, F.; Saucedo, A.; Flores-Solis, D.

Citation: Saucedo, Alma Leticia; Flores-Solis, David; Rodriguez de la Vega, Ricardo; Ramirez-Cordero, Belen; Hernandez-Lopez, Rogelio; Cano-Sanchez, Patricia; Noriega Navarro, Roxana; Garcia-Valdes, Jesus; Coronas-Valderrama, Fredy; de Roodt, Adolfo; Brieba, Luis; Domingos Possani, Lourival; del Rio-Portilla, Federico. "New tricks of an old pattern: structural versatility of scorpion toxins with common cysteine spacing."  J. Biol. Chem. 287, 12321-12330 (2012).

Assembly members:
Ts16, polymer, 31 residues, 3460.127 Da.

Natural source:   Common Name: Brazilian scorpion   Taxonomy ID: 6887   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Tityus serrulatus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ts16: GSGCMKEYCAGQCRGKVSQD YCLKHCKCIPR

Data sets:
Data typeCount
1H chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ts161

Entities:

Entity 1, Ts16 31 residues - 3460.127 Da.

1   GLYSERGLYCYSMETLYSGLUTYRCYSALA
2   GLYGLNCYSARGGLYLYSVALSERGLNASP
3   TYRCYSLEULYSHISCYSLYSCYSILEPRO
4   ARG

Samples:

sample: Ts16 1.4 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQFCOSYsampleisotropicsample_conditions_1
TOCSYsampleisotropicsample_conditions_1
NOESYsampleisotropicsample_conditions_1
NOESYsampleisotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian VNMRS 700 MHz

Related Database Links:

PDB
SP P86271