BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18014

Title: A Myristoylated Polyproline Type II Helix Functions as a Novel Fusion Peptide During Cell-Cell Membrane Fusion Induced by the Baboon Reovirus p15 FAST Protein

Deposition date: 2011-10-21 Original release date: 2011-11-29

Authors: Top, Deniz; Read, Jolene; Dawe, Sandra; Syvitski, Raymond; Duncan, Roy

Citation: Top, Deniz; Read, Jolene; Dawe, Sandra; Syvitski, Raymond; Duncan, Roy. "A Myristoylated Polyproline Type II Helix Functions as a Novel Fusion Peptide During Cell-Cell Membrane Fusion Induced by the Baboon Reovirus p15 FAST Protein"  J. Biol. Chem. ., .-..

Assembly members:
entity, polymer, 20 residues, 2156.466 Da.

Natural source:   Common Name: Orthoreovirus   Taxonomy ID: 10882   Superkingdom: Viruses   Kingdom: not available   Genus/species: Orthoreovirus not available

Experimental source:   Production method: recombinant technology   Host organism: Desulfovibrio desulfuricans

Entity Sequences (FASTA):
entity: GQRHSIVQPPAPPPNAFVEI

Data sets:
Data typeCount
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Myristoylated Polyproline Type II Helix1

Entities:

Entity 1, Myristoylated Polyproline Type II Helix 20 residues - 2156.466 Da.

1   GLYGLNARGHISSERILEVALGLNPROPRO
2   ALAPROPROPROASNALAPHEVALGLUILE

Samples:

sample_1: entity 5 ± 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY v3.1, Goddard - chemical shift assignment, data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization, refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAL01373
REF YP_004769555