BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18063

Title: NH chemical shift Assignments for AbpSH3 bound to mutant ArkA peptide (L(-7)A)   PubMed: 23251481

Deposition date: 2011-11-13 Original release date: 2013-01-04

Authors: Stollar, Elliott; Forman-Kay, Julie

Citation: Stollar, Elliott; Lin, Hong; Davidson, Alan; Forman-Kay, Julie. "Differential Dynamic Engagement within 24 SH3 Domain: Peptide Complexes Revealed by Co-Linear Chemical Shift Perturbation Analysis."  PLoS ONE 7, e51282-e51282 (2012).

Assembly members:
AbpSH3, polymer, 59 residues, Formula weight is not available
ArkA_L(-7)A, polymer, 17 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
AbpSH3: APWATAEYDYDAAEDNELTF VENDKIINIEFVDDDWWLGE LEKDGSKGLFPSNYVSLGN
ArkA_L(-7)A: KKTKPTPPPKPSHAKPK

Data sets:
Data typeCount
15N chemical shifts64
1H chemical shifts64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AbpSH31
2ArkA_L(-7)A2

Entities:

Entity 1, AbpSH3 59 residues - Formula weight is not available

1   ALAPROTRPALATHRALAGLUTYRASPTYR
2   ASPALAALAGLUASPASNGLULEUTHRPHE
3   VALGLUASNASPLYSILEILEASNILEGLU
4   PHEVALASPASPASPTRPTRPLEUGLYGLU
5   LEUGLULYSASPGLYSERLYSGLYLEUPHE
6   PROSERASNTYRVALSERLEUGLYASN

Entity 2, ArkA_L(-7)A 17 residues - Formula weight is not available

1   LYSLYSTHRLYSPROTHRPROPROPROLYS
2   PROSERHISALALYSPROLYS

Samples:

AbpSH3-ArkA_L(-7)A: AbpSH3, [U-100% 15N], 1900 uM; sodium phosphate 50 mM; sodium chloride 100 mM; D2O 10%; H2O 90%; ArkA_L(-7)A 4000 uM

sample_conditions_1: ionic strength: 0.189 M; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCAbpSH3-ArkA_L(-7)Aisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard, Johnson, One Moon Scientific - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 16843 18054 18055 18056 18057 18058 18059 18060 18061 18062 18064 18065 18066 18067 18068 18069 18070 18071 18072 18073 18074 18075 18076 18077 18078 25354 26519
PDB
DBJ GAA21988
EMBL CAA36075 CAA42253 CAY78291
GB AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991
PRF 1603360A
REF NP_010012
SP P15891
TPG DAA07557

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