BMRB Entry 18078
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18078
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Title: NH chemical shift Assignments for AbpSH3 bound to WT Srv2 peptide (Srv17) PubMed: 23251481
Deposition date: 2011-11-13 Original release date: 2013-01-03
Authors: Stollar, Elliott; Forman-Kay, Julie
Citation: Stollar, Elliott; Lin, Hong; Davidson, Alan; Forman-Kay, Julie. "Differential Dynamic Engagement within 24 SH3 Domain: Peptide Complexes Revealed by Co-Linear Chemical Shift Perturbation Analysis." PLoS ONE 7, e51282-e51282 (2012).
Assembly members:
AbpSH3, polymer, 59 residues, Formula weight is not available
Srv17, polymer, 17 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
AbpSH3: APWATAEYDYDAAEDNELTF
VENDKIINIEFVDDDWWLGE
LEKDGSKGLFPSNYVSLGN
Srv17: KSGPPPRPKKPSTLKTK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 65 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AbpSH3 | 1 |
| 2 | Srv17 | 2 |
Entities:
Entity 1, AbpSH3 59 residues - Formula weight is not available
| 1 | ALA | PRO | TRP | ALA | THR | ALA | GLU | TYR | ASP | TYR | ||||
| 2 | ASP | ALA | ALA | GLU | ASP | ASN | GLU | LEU | THR | PHE | ||||
| 3 | VAL | GLU | ASN | ASP | LYS | ILE | ILE | ASN | ILE | GLU | ||||
| 4 | PHE | VAL | ASP | ASP | ASP | TRP | TRP | LEU | GLY | GLU | ||||
| 5 | LEU | GLU | LYS | ASP | GLY | SER | LYS | GLY | LEU | PHE | ||||
| 6 | PRO | SER | ASN | TYR | VAL | SER | LEU | GLY | ASN |
Entity 2, Srv17 17 residues - Formula weight is not available
| 1 | LYS | SER | GLY | PRO | PRO | PRO | ARG | PRO | LYS | LYS | ||||
| 2 | PRO | SER | THR | LEU | LYS | THR | LYS |
Samples:
AbpSH3-Srv17: AbpSH3, [U-100% 15N], 100 uM; sodium phosphate 50 mM; sodium chloride 100 mM; D2O 10%; H2O 90%; Srv17 600 uM
sample_conditions_1: ionic strength: 0.189 M; pH: 7; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | AbpSH3-Srv17 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard, Johnson, One Moon Scientific - chemical shift assignment, data analysis
NMR spectrometers:
- Varian Unity 500 MHz
Related Database Links:
| BMRB | 16843 18054 18055 18056 18057 18058 18059 18060 18061 18062 18063 18064 18065 18066 18067 18068 18069 18070 18071 18072 18073 18074 18075 18076 18077 25354 26519 |
| PDB | |
| DBJ | GAA21988 GAA25960 |
| EMBL | CAA36075 CAA42253 CAY78291 CAA86887 CAA96020 CAY82465 |
| GB | AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991 AAA35094 AAA63569 AHY76960 EDN62677 EDV12390 |
| PRF | 1603360A |
| REF | NP_010012 NP_014261 XP_011105209 |
| SP | P15891 P17555 |
| TPG | DAA07557 DAA10410 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts