BMRB Entry 18148
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18148
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Title: VEGFR2-Integrin B3 interaction PubMed: 22363548
Deposition date: 2011-12-18 Original release date: 2012-03-02
Authors: Deshmukh, Lalit; Vinogradova, Olga
Citation: West, Xiaoxia; Meller, Nahum; Malinin, Nikolay; Deshmukh, Lalit; Meller, Julia; Mahabeleshwar, Ganapati; Weber, Malory; Kerr, Bethany; Vinogradova, Olga; Byzova, Tatiana. "Integrin (3) Crosstalk with VEGFR Accommodating Tyrosine Phosphorylation as a Regulatory Switch." PLoS ONE 7, .-. (2012).
Assembly members:
cytoplasmic_tail_of_VEGFR-2, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
cytoplasmic_tail_of_VEGFR-2: LRTVKRANGGELKTGYLSIV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 58 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VEGFR-2 | 1 |
Entities:
Entity 1, VEGFR-2 20 residues - Formula weight is not available
| 1 | LEU | ARG | THR | VAL | LYS | ARG | ALA | ASN | GLY | GLY | |
| 2 | GLU | LEU | LYS | THR | GLY | TYR | LEU | SER | ILE | VAL |
Samples:
sample_1: cytoplasmic tail of VEGFR-2 1 mM; D2O 5%; H20 95%; DSS 1 mM; sodium chloride 50 mM; sodium phosphate 25 mM; GST-tagged B3 integrin tail 20 uM
sample_conditions_1: pH: 6.1; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH v2.24, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD93138 |
| EMBL | CAO79613 |
| GB | AAB88005 AAC16450 AAI31823 ABB82619 ACF37106 |
| REF | NP_001041489 NP_001103470 NP_001182599 NP_001265494 NP_002244 |
| SP | P35968 |
| TPG | DAA28658 |