BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 18339

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18339

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Title: 1H, 15N and 13C backbone resonance assignments of FTSA   PubMed: 22473211

Deposition date: 2012-03-20 Original release date: 2012-04-11

Authors: Szwedziak, Piotr; Wang, Qing; Freund, Stefan; Lowe, Jan

Citation: Szwedziak, Piotr; Wang, Qing; Freund, Stefan Mv; Lowe, Jan. "FtsA forms actin-like protofilaments."  EMBO J. 31, 2249-2260 (2012).

Assembly members:
FTSA, polymer, 419 residues, Formula weight is not available

Natural source:   Common Name: Thermotogales   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FTSA: MIDLSKTVFYTSIDIGSRYI KGLVLGKRDQEWEALAFSSV KSRGLDEGEIKDAIAFKESV NTLLKELEEQLQKSLRSDFV ISFSSVSFEREDTVIERDFG EEKRSITLDILSEMQSEALE KLKENGKTPLHIFSKRYLLD DERIVFNPLDMKASKIAIEY TSIVVPLKVYEMFYNFLQDT VKSPFQLKSSLVSTAEGVLT TPEKDRGVVVVNLGYNFTGL IAYKNGVPIKISYVPVGMKH VIKDVSAVLDTSFEESERLI ITHGNAVYNDLKEEEIQYRG LDGNTIKTTTAKKLSVIIHA RLREIMSKSKKFFREVEAKI VEEGEIGIPGGVVLTGGGAK IPRINELATEVFKSPVRTGC YANSDRPSIINADEVANDPS FAAAFGNVFAVSENPYEETP VKSENPLKKIFRLFKELME

Data sets:
Data typeCount
13C chemical shifts1049
15N chemical shifts324
1H chemical shifts324

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FTSA1

Entities:

Entity 1, FTSA 419 residues - Formula weight is not available

1   METILEASPLEUSERLYSTHRVALPHETYR
2   THRSERILEASPILEGLYSERARGTYRILE
3   LYSGLYLEUVALLEUGLYLYSARGASPGLN
4   GLUTRPGLUALALEUALAPHESERSERVAL
5   LYSSERARGGLYLEUASPGLUGLYGLUILE
6   LYSASPALAILEALAPHELYSGLUSERVAL
7   ASNTHRLEULEULYSGLULEUGLUGLUGLN
8   LEUGLNLYSSERLEUARGSERASPPHEVAL
9   ILESERPHESERSERVALSERPHEGLUARG
10   GLUASPTHRVALILEGLUARGASPPHEGLY
11   GLUGLULYSARGSERILETHRLEUASPILE
12   LEUSERGLUMETGLNSERGLUALALEUGLU
13   LYSLEULYSGLUASNGLYLYSTHRPROLEU
14   HISILEPHESERLYSARGTYRLEULEUASP
15   ASPGLUARGILEVALPHEASNPROLEUASP
16   METLYSALASERLYSILEALAILEGLUTYR
17   THRSERILEVALVALPROLEULYSVALTYR
18   GLUMETPHETYRASNPHELEUGLNASPTHR
19   VALLYSSERPROPHEGLNLEULYSSERSER
20   LEUVALSERTHRALAGLUGLYVALLEUTHR
21   THRPROGLULYSASPARGGLYVALVALVAL
22   VALASNLEUGLYTYRASNPHETHRGLYLEU
23   ILEALATYRLYSASNGLYVALPROILELYS
24   ILESERTYRVALPROVALGLYMETLYSHIS
25   VALILELYSASPVALSERALAVALLEUASP
26   THRSERPHEGLUGLUSERGLUARGLEUILE
27   ILETHRHISGLYASNALAVALTYRASNASP
28   LEULYSGLUGLUGLUILEGLNTYRARGGLY
29   LEUASPGLYASNTHRILELYSTHRTHRTHR
30   ALALYSLYSLEUSERVALILEILEHISALA
31   ARGLEUARGGLUILEMETSERLYSSERLYS
32   LYSPHEPHEARGGLUVALGLUALALYSILE
33   VALGLUGLUGLYGLUILEGLYILEPROGLY
34   GLYVALVALLEUTHRGLYGLYGLYALALYS
35   ILEPROARGILEASNGLULEUALATHRGLU
36   VALPHELYSSERPROVALARGTHRGLYCYS
37   TYRALAASNSERASPARGPROSERILEILE
38   ASNALAASPGLUVALALAASNASPPROSER
39   PHEALAALAALAPHEGLYASNVALPHEALA
40   VALSERGLUASNPROTYRGLUGLUTHRPRO
41   VALLYSSERGLUASNPROLEULYSLYSILE
42   PHEARGLEUPHELYSGLULEUMETGLU

Samples:

sample_1: FTSA, [U-100% 13C; U-100% 15N; U-100% 2H], 85 uM; H2O 95%; FTSA 5%

sample_conditions_1: ionic strength: 33 mM; pH: 7.5; pressure: 1 atm; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY-HSQCsample_1isotropicsample_conditions_1
3D TROSY-HNCAsample_1isotropicsample_conditions_1
3D TROSY-HN(CO)CAsample_1isotropicsample_conditions_1
3D TROSY-HNCACBsample_1isotropicsample_conditions_1
3D TROSY-HN(COCA)CBsample_1isotropicsample_conditions_1
3D TROSY-HN(CA)COsample_1isotropicsample_conditions_1
3D TROSY-HNCOsample_1isotropicsample_conditions_1

Software:

SPARKY v3.110, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
GB AAD35917 ABQ46121 ACB08454 ADA66200 AGL49762
REF NP_228644 WP_004080802 WP_008193022 WP_010865211 WP_011942795

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts