BMRB Entry 18403
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18403
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Title: 1H,13C and 15N resonance assignment of the UIM-SH3 construct of the STAM2 protein PubMed: 22841719
Deposition date: 2012-04-16 Original release date: 2012-07-17
Authors: Lange, Anja; Ismail, Mouhamad-Baligh; Riviere, Gwladys; Hologne, Maggy; Guilliere, Florence; Lancelin, Jean-Marc; Krimm, Isabelle; Walker, Olivier
Citation: Lange, Anja; Ismail, Mouhamad-Baligh; Riviere, Gwladys; Hologne, Maggy; Lacabanne, Denis; Guilliere, Florence; Lancelin, Jean-Marc; Krimm, Isabelle; Walker, Olivier. "Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR." FEBS Lett. 586, 3379-3384 (2012).
Assembly members:
UIM-SH3_domains_of_STAM2, polymer, 109 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
UIM-SH3_domains_of_STAM2: GAMGMNKNKEDEDIAKAIEL
SLQEQKQQHTETKSLYPSSE
IQLNNKVARKVRALYDFEAV
EDNELTFKHGEIIIVLDDSD
ANWWKGENHRGIGLFPSDFV
TTNLNIETE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 194 |
| 15N chemical shifts | 104 |
| 1H chemical shifts | 104 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UIM-SH3 monomer | 1 |
Entities:
Entity 1, UIM-SH3 monomer 109 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | MET | ASN | LYS | ASN | LYS | GLU | ||||
| 2 | ASP | GLU | ASP | ILE | ALA | LYS | ALA | ILE | GLU | LEU | ||||
| 3 | SER | LEU | GLN | GLU | GLN | LYS | GLN | GLN | HIS | THR | ||||
| 4 | GLU | THR | LYS | SER | LEU | TYR | PRO | SER | SER | GLU | ||||
| 5 | ILE | GLN | LEU | ASN | ASN | LYS | VAL | ALA | ARG | LYS | ||||
| 6 | VAL | ARG | ALA | LEU | TYR | ASP | PHE | GLU | ALA | VAL | ||||
| 7 | GLU | ASP | ASN | GLU | LEU | THR | PHE | LYS | HIS | GLY | ||||
| 8 | GLU | ILE | ILE | ILE | VAL | LEU | ASP | ASP | SER | ASP | ||||
| 9 | ALA | ASN | TRP | TRP | LYS | GLY | GLU | ASN | HIS | ARG | ||||
| 10 | GLY | ILE | GLY | LEU | PHE | PRO | SER | ASP | PHE | VAL | ||||
| 11 | THR | THR | ASN | LEU | ASN | ILE | GLU | THR | GLU |
Samples:
sample_1: UIM-SH3 domains of STAM2, [U-13C; U-15N], 200-400 uM; sodium phosphate 20 mM; sodium azide 0.02%
sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAF84954 BAF85536 BAG72771 BAK62690 |
| EMBL | CAB63735 CAL38297 |
| GB | AAC63963 AAC63964 AAH28740 AAY14712 ABM83353 |
| REF | NP_001267210 NP_005834 XP_003275024 XP_003776054 XP_003832773 |
| SP | O75886 |
Download simulated HSQC data in one of the following formats:
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